Biochemical and Biophysical Research Communications 2013-04-05

A small molecule inhibitor of Bcl-2, HA14-1, also inhibits ceramide glucosyltransferase.

Satomi Niino, Yutaka Nakamura, Yoshio Hirabayashi, Michiyo Nagano-Ito, Shinichi Ichikawa

Index: Biochem. Biophys. Res. Commun. 433(2) , 170-4, (2013)

Full Text: HTML

Abstract

HA14-1 is a Bcl-2 inhibitor that is widely used for studies of apoptosis. In the course of searching for a ceramide glucosyltransferase inhibitor that catalyzes the first glycosylation step of glycosphingolipid synthesis, we unexpectedly found that HA-14-1 also has the ability to inhibit ceramide glucosyltransferase. The IC50 value of HA14-1 against ceramide glucosyltransferase is 4.5μM, which is lower than that reported for Bcl-2 in vitro. Kinetic analyses revealed that HA14-1 is a competitive and mixed-type inhibitor with respect to C6-NBD-ceramide and UDP-glucose, respectively.Copyright © 2013 Elsevier Inc. All rights reserved.

Related Compounds

  • 4-Chloro-7-nitrobe...
  • N-{6-[(7-nitro-2,1...

Related Articles:

Cytotoxic bile acids, but not cytoprotective species, inhibit the ordering effect of cholesterol in model membranes at physiologically active concentrations.

2013-09-01

[Biochim. Biophys. Acta 1828(9) , 2152-63, (2013)]

Evaluation of a microchip electrophoresis-mass spectrometry platform deploying a pressure-driven make-up flow.

2013-04-12

[J. Chromatogr. A. 1285 , 159-64, (2013)]

Determination of hinokitiol in skin lotion by high-performance liquid chromatography-ultraviolet detection after precolumn derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole.

2013-01-01

[J. Cosmet. Sci. 64(5) , 381-9, (2013)]

Determination of kojic acid in a skin-whitening cosmetic by high-performance liquid chromatography coupled with ultraviolet detection after pre-column derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole.

2012-01-01

[J. Cosmet. Sci. 63(3) , 205-12, (2012)]

Simple and sensitive high performance liquid chromatography method with fluorescence detection for therapeutic drug monitoring of topiramate.

2015-01-01

[Acta Chim. Slov. 62 , 411-9, (2015)]

More Articles...