steroids

Xanthomonas maltophilia CBS 897.97 as a source of new 7β-and 7α-hydroxysteroid dehydrogenases and cholylglycine hydrolase: improved biotransformations of …

P Pedrini, E Andreotti, A Guerrini, M Dean, G Fantin…

Index: Pedrini, Paola; Andreotti, Elisa; Guerrini, Alessandra; Dean, Mariangela; Fantin, Giancarlo; Giovannini, Pier Paolo Steroids, 2006 , vol. 71, # 3 p. 189 - 198

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Citation Number: 21

Abstract

The paper reports the partial purification and characterization of the 7β-and 7α- hydroxysteroid dehydrogenases (HSDH) and cholylglycine hydrolase (CGH), isolated from Xanthomonas maltophilia CBS 897.97. The activity of 7β-HSDH and 7α-HSDH in the reduction of the 7-keto bile acids is determined. The affinity of 7β-HSDH for bile acids is confirmed by the reduction, on analytical scale, to the corresponding 7β-OH derivatives. A ...

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