Journal of Pharmaceutical and Biomedical Analysis 1996-06-01

Analysis of steroids Part 50. Derivatization of ketosteroids for their separation and determination by capillary electrophoresis.

S Görög, M Gazdag, P Kemenes-Bakos

Index: J. Pharm. Biomed. Anal. 14(8-10) , 1115-24, (1996)

Full Text: HTML

Abstract

4-Ene-3-ketosteroids and 17-ketosteroids were quantitatively transformed into the corresponding hydrazones using Girard P and T reagents, respectively. The positively charged derivatives were separated by capillary electrophoresis. The spectrophotometric characteristics of the derivatives permitted their sensitive detection in the 230-280 nm range. The steroids investigated included nortestosterone and its phenylpropionate, norethisterone and its oenanthate, d,l-norgestrel, dehydroepiandrosterone, androstenedione and ethisterone.

Related Compounds

  • Girard's reagent...

Related Articles:

Derivatization with Girard reagent T combined with LC-MS/MS for the sensitive detection of 5-formyl-2'-deoxyuridine in cellular DNA.

2007-01-01

[Anal. Chem. 79(1) , 322-6, (2007)]

Girard derivatization for LC-MS/MS profiling of endogenous ecdysteroids in Drosophila.

2013-08-01

[J. Lipid Res. 54(8) , 2265-72, (2013)]

Derivatization of small oligosaccharides prior to analysis by matrix-assisted laser desorption/ionization using glycidyltrimethylammonium chloride and Girard's reagent T.

2002-01-01

[Rapid Commun. Mass Spectrom. 16(10) , 905-12, (2002)]

Reactions of microsolvated organic compounds at ambient surfaces: droplet velocity, charge state, and solvent effects.

2012-06-01

[J. Am. Soc. Mass Spectrom. 23(6) , 1077-84, (2012)]

A modified Girard derivatizing reagent for universal profiling and trace analysis of aldehydes and ketones by electrospray ionization tandem mass spectrometry.

2007-01-01

[Rapid Commun. Mass Spectrom. 21(18) , 2926-32, (2007)]

More Articles...