Girard's reagent T
Girard's reagent T structure
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Common Name | Girard's reagent T | ||
|---|---|---|---|---|
| CAS Number | 123-46-6 | Molecular Weight | 167.637 | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | C5H14ClN3O | Melting Point | 188-192 °C (dec.)(lit.) | |
| MSDS | Chinese USA | Flash Point | N/A | |
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Derivatization with Girard reagent T combined with LC-MS/MS for the sensitive detection of 5-formyl-2'-deoxyuridine in cellular DNA.
Anal. Chem. 79(1) , 322-6, (2007) Nucleoside 5-formyl-2'-deoxyuridine (FodU) is a major thymidine lesion generated by reactive oxygen species. In vitro and in vivo replication studies revealed that FodU can be mutagenic. A reliable and sensitive quantification method is, therefore, important ... |
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Girard derivatization for LC-MS/MS profiling of endogenous ecdysteroids in Drosophila.
J. Lipid Res. 54(8) , 2265-72, (2013) Ecdysteroids are potent developmental regulators that control molting, reproduction, and stress response in arthropods. In developing larvae, picogram quantities of individual ecdysteroids and their conjugated forms are present along with milligrams of struct... |
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Derivatization of small oligosaccharides prior to analysis by matrix-assisted laser desorption/ionization using glycidyltrimethylammonium chloride and Girard's reagent T.
Rapid Commun. Mass Spectrom. 16(10) , 905-12, (2002) In matrix-assisted laser desorption/ionization (MALDI) analyses of small oligosaccharides a very large increase in sensitivity (by a factor of 1000) may be obtained by introducing a quaternary ammonium center ('quaternization'). Such a quaternary ammonium cen... |
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Reactions of microsolvated organic compounds at ambient surfaces: droplet velocity, charge state, and solvent effects.
J. Am. Soc. Mass Spectrom. 23(6) , 1077-84, (2012) The exposure of charged microdroplets containing organic ions to solid-phase reagents at ambient surfaces results in heterogeneous ion/surface reactions. The electrosprayed droplets were driven pneumatically in ambient air and then electrically directed onto ... |
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A modified Girard derivatizing reagent for universal profiling and trace analysis of aldehydes and ketones by electrospray ionization tandem mass spectrometry.
Rapid Commun. Mass Spectrom. 21(18) , 2926-32, (2007) 4-Hydrazino-N,N,N-trimethyl-4-oxobutanaminium iodide (HTMOB) is a modified Girard derivatizing reagent synthesized to improve the sensitivity of analysis of aldehydes and ketones with electrospray ionization tandem mass spectrometry. Compared with Girard T re... |
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Unique potentiometric detection systems for HPLC determination of some steroids in human urine.
J. Sep. Sci. 32(2) , 167-79, (2009) Isocratic HPLC with potentiometric detection is used for the determination of some 17-ketosteroids (17-KS), e.g., androsterone, dehydroepiandrosterone and estrone, and their respective sulfated conjugates (17-KSS). Glassy carbon or composite electrodes contai... |
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Analysis of succinylacetone, as a Girard T derivative, in urine and dried bloodspots by flow injection electrospray ionization tandem mass spectrometry.
Rapid Commun. Mass Spectrom. 21(1) , 59-63, (2007) Flow injection electrospray ionization tandem mass spectrometric methods for succinylacetone (SA) in 250 microL urine, using d5-SA as internal standard, and in 3 mm dried bloodspots, using 13C4-SA as internal standard, are described. Selectivity and sensitivi... |
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The participation of trimethylammonium in the mouse erythrocyte epitope recognized by monoclonal autoantibodies.
Immunol. Lett. 3(5) , 315-9, (1981) Monoclonal IgM anti-erythrocyte autoantibody produced by a NZB-derived hybridoma has been found to react with trimethylammonium-containing compounds. Such compounds are able to prevent the lysis of bromelain-treated mouse erythrocytes (BrMRBC) by those autoan... |
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Mass spectrometric quantification of neutral and sialylated N-glycans from a recombinant therapeutic glycoprotein produced in the two Chinese hamster ovary cell lines.
Anal. Biochem. 386(2) , 228-36, (2009) Quality control and assurance of glycan profiles of a recombinant glycoprotein from lot to lot is a critical issue in the pharmaceutical industry. To develop an easy and simple quantitative and qualitative glycan profile method based on matrix-assisted laser ... |
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Analysis of steroids Part 50. Derivatization of ketosteroids for their separation and determination by capillary electrophoresis.
J. Pharm. Biomed. Anal. 14(8-10) , 1115-24, (1996) 4-Ene-3-ketosteroids and 17-ketosteroids were quantitatively transformed into the corresponding hydrazones using Girard P and T reagents, respectively. The positively charged derivatives were separated by capillary electrophoresis. The spectrophotometric char... |