Journal of Chromatography B 2015-03-01

Simultaneous determination of six alkaloid components in rat plasma and its application to pharmacokinetic study of Danmu preparations by an ultra fast liquid chromatography-electrospray ionization-tandem mass spectrometry.

Rong Yin, Jiaquan Chen, Yonggang Zhao, Xiaobin Jia, Zhiyuan Zhang, Liang Feng, Hui Wang, Jingjing Wang, Fenxia Zhu

文献索引:J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 983-984 , 10-7, (2015)

全文:HTML全文

摘要

Danmu injection and Danmu tablet are two widely used traditional Chinese medicine made of Nauclea officinalis (commonly known as Danmu), in which the alkaloids are the major active substances. In this paper, an ultra fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) method was developed for simultaneous determination and the pharmacokinetic characteristics study of six main active alkaloids (naucleamide A-10-O-β-d-glucopyranosid, naucleamide G, pumiloside, 3-epi-pumiloside, strictosamide and vincosamide) of the two above-mentioned Danmu preparations in rat plasma. In the course of the experiment, following sample preparation by protein precipitation with methanol-ethyl acetate (2:1, v/v), the nitrogen-dried extraction was reconstituted in methanol and assayed on a C18 column using a gradient elution program with mobile phase consisting of acetonitrile and water containing 0.1% formic acid. The MS detection was performed in positive ionization mode with selected ion transitions. The established method was fully validated and proved to be sensitive and specific with lower limits of quantification (LLOQs) all less than 0.32ng/mL in rat plasma and matrix effects ranged from 88.87 to 108.27%. Good linearities of six alkaloids were obtained in respective concentration ranges (r(2)>0.995). The average extract recoveries for each compound at three quality control concentration levels were no less than 79.70%, and the precision and accuracy were within the acceptable limits. The validated method was successfully applied to the pharmacokinetic study of six alkaloid components of Danmu injection and tablet in rat plasma. The obtained results may be helpful to reveal the action mechanism and guide the clinical application of Danmu preparations. Copyright © 2015 Elsevier B.V. All rights reserved.


相关化合物

  • 甲酸
  • 乙腈
  • 甲醇
  • 喜果苷

相关文献:

Neuroprotective effect of modified Chungsimyeolda-tang, a traditional Korean herbal formula, via autophagy induction in models of Parkinson's disease.

2015-01-15

[J. Ethnopharmacol. 159 , 93-101, (2014)]

Biomolecular imaging with a C60-SIMS/MALDI dual ion source hybrid mass spectrometer: instrumentation, matrix enhancement, and single cell analysis.

2014-11-01

[J. Am. Soc. Mass Spectrom. 25(11) , 1897-907, (2014)]

Methionine oxidation accelerates the aggregation and enhances the neurotoxicity of the D178N variant of the human prion protein.

2014-12-01

[Biochim. Biophys. Acta 1842(12 Pt A) , 2345-56, (2014)]

Quantification of furanic derivatives in fortified wines by a highly sensitive and ultrafast analytical strategy based on digitally controlled microextraction by packed sorbent combined with ultrahigh pressure liquid chromatography.

2015-02-13

[J. Chromatogr. A. 1381 , 54-63, (2015)]

Silencing urease: a key evolutionary step that facilitated the adaptation of Yersinia pestis to the flea-borne transmission route.

2014-12-30

[Proc. Natl. Acad. Sci. U. S. A. 111(52) , 18709-14, (2014)]

更多文献...