Journal of chromatography. A 2015-04-17

Development of amino acid derivatization reagents for liquid chromatography electrospray ionization mass spectrometric analysis and ionization efficiency measurements.

Riin Rebane, Toomas Rodima, Agnes Kütt, Koit Herodes

文献索引：J. Chromatogr. A. 1390 , 62-70, (2015)

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摘要

Derivatization is one of the most common ways for improving chromatographic separation and sensitivity for LC-ESI-MS analysis. The aim of this work was to design new derivatization reagents for LC-ESI-MS analysis of amino acids which would (1) provide good reversed phase chromatographic separation, (2) most importantly, provide low detection limits, (3) be easily synthesized, (4) produce derivatives which are less susceptible to matrix influences and (5) have convenient derivatization procedure with stable derivatives suitable for automatization. In the current work two new LC-ESI-MS compatible derivatization reagents have been designed and synthesized, dibenzyl ethoxymethylene malonate (DBEMM) and benzyl ethyl ethoxymethylene malonate (EBEMM). The DBEMM meets all the goals set with instrumental detection limits as low as 1 femtomole for amino acids and 40 attomole for selenoamino acids. Copyright © 2015 Elsevier B.V. All rights reserved.

相关化合物

  • 甲酸
  • 乙腈
  • 乙酸酐
  • 二乙烯苯
  • 巯基乙醇
  • 二苄基丙二酸酯
  • 六氟异丙醇

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