Journal of Agricultural and Food Chemistry 2014-12-31

Molecular characterization of a thermophilic endo-polygalacturonase from Thielavia arenaria XZ7 with high catalytic efficiency and application potential in the food and feed industries.

Tao Tu, Kun Meng, Huoqing Huang, Huiying Luo, Yingguo Bai, Rui Ma, Xiaoyun Su, Pengjun Shi, Peilong Yang, Yaru Wang, Bin Yao

文献索引：J. Agric. Food Chem. 62(52) , 12686-94, (2015)

全文：HTML全文

摘要

Thermophilic endo-polygalacturonases with high catalytic efficiency are of great interest in the food and feed industries. This study identified an endo-polygalacturonase gene (pg7fn) of glycoside hydrolase family 28 in the thermophilic fungus Thielavia arenaria XZ7. Recombinant PG7fn produced in Pichia pastoris is distinguished from other enzyme counterparts by its high functional temperature (60 °C) and specific activity (34382 ± 351 U/mg toward polygalacturonic acid). The enzyme exhibited good pH stability (pH 3.0-8.0) and resistance to pepsin and trypsin digestion and had a significant effect on disaggregation of soybean meal. Addition of 1 U/g PG7fn increased the pectin bioavailability by 19.33%. The excellent properties described above make PG7fn valuable for applications in the food and feed industries. Furthermore, a comparative study showed that N-glycosylation improved the thermostability and catalytic efficiency of PG7fn.

相关化合物

  • 甲醇
  • 三半乳糖醛酸
  • L-甲状腺素钠五水合...
  • 半双乳糖醛酸
  • 左旋甲状腺素

相关文献：

BBS4 directly affects proliferation and differentiation of adipocytes.

2014-09-01

[Cell. Mol. Life Sci. 71(17) , 3381-92, (2014)]

Urinary metabolic fingerprinting of mice with diet-induced metabolic derangements by parallel dual secondary column-dual detection two-dimensional comprehensive gas chromatography.

2014-09-26

[J. Chromatogr. A. 1361 , 265-76, (2014)]

Biomolecular imaging with a C60-SIMS/MALDI dual ion source hybrid mass spectrometer: instrumentation, matrix enhancement, and single cell analysis.

2014-11-01

[J. Am. Soc. Mass Spectrom. 25(11) , 1897-907, (2014)]

Synergistic activity of tenofovir and nevirapine combinations released from polycaprolactone matrices for potential enhanced prevention of HIV infection through the vaginal route.

2014-10-01

[Eur. J. Pharm. Biopharm. 88(2) , 406-14, (2014)]

Antinematode activity of Violacein and the role of the insulin/IGF-1 pathway in controlling violacein sensitivity in Caenorhabditis elegans.

2014-01-01

[PLoS ONE 9(10) , e109201, (2014)]

更多文献...