Two-step, PCR-free telomerase detection by using exonuclease III-aided target recycling.
Xiaolei Zuo, Fan Xia, Adriana Patterson, H Tom Soh, Yi Xiao, Kevin W Plaxco
文献索引:ChemBioChem. 12(18) , 2745-7, (2011)
全文:HTML全文
摘要
We report the sensitive detection of telomerase activity by using exonuclease III-aided target recycling to amplify the signal produced by a chimeric LNA-DNA molecular beacon. We demonstrate the specific detection of as few as 30 telomerase-positive breast cancer cells in a single-measurement fluorescence assay that avoids the problematic PCR and gel analysis of the current "gold-standard" assay.Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
相关化合物
相关文献:
2013-06-12
[J. Mol. Biol. 425(11) , 1946-60, (2013)]
2012-05-21
[Anal. Chim. Acta 727 , 67-70, (2012)]
2013-01-25
[Anal. Chim. Acta 761 , 137-42, (2013)]
2012-11-15
[Talanta 101 , 357-61, (2012)]
2012-01-25
[Chem. Commun. (Camb.) 48(7) , 1018-20, (2012)]