外切核酸酶III
外切核酸酶III结构式
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常用名 | 外切核酸酶III | 英文名 | ExonucleaseIII |
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| CAS号 | 9037-44-9 | 分子量 | N/A | |
| 密度 | N/A | 沸点 | N/A | |
| 分子式 | N/A | 熔点 | N/A | |
| MSDS | 中文版 美版 | 闪点 | N/A |
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Combined micrococcal nuclease and exonuclease III digestion reveals precise positions of the nucleosome core/linker junctions: implications for high-resolution nucleosome mapping.
J. Mol. Biol. 425(11) , 1946-60, (2013) Micrococcal nuclease (MNase) is extensively used in genome-wide mapping of nucleosomes but its preference for AT-rich DNA leads to errors in establishing precise positions of nucleosomes. Here, we show that the MNase digestion of nucleosomes assembled on a st... |
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Graphene oxide-based biosensor for sensitive fluorescence detection of DNA based on exonuclease III-aided signal amplification.
Anal. Chim. Acta 727 , 67-70, (2012) Based on the super fluorescence quenching efficiency of graphene oxide and exonuclease III aided signal amplification, we develop a facile, sensitive, rapid and cost-effective method for DNA detection. In the presence of target DNA, the target-probe hybridiza... |
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Two-step, PCR-free telomerase detection by using exonuclease III-aided target recycling.
ChemBioChem. 12(18) , 2745-7, (2011) We report the sensitive detection of telomerase activity by using exonuclease III-aided target recycling to amplify the signal produced by a chimeric LNA-DNA molecular beacon. We demonstrate the specific detection of as few as 30 telomerase-positive breast ca... |
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Sensitive chemiluminescence aptasensor based on exonuclease-assisted recycling amplification.
Anal. Chim. Acta 761 , 137-42, (2013) We report herein an exonuclease-assisted aptamer-based target recycling amplification strategy for sensitive and selective chemiluminescence (CL) determination of adenosine. This aptasensor is based on target-induced release of aptamers from capture probes im... |
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A novel exonuclease III-aided amplification assay for lysozyme based on graphene oxide platform.
Talanta 101 , 357-61, (2012) Based on exonuclease III (Exo III) aided amplification and graphene oxide (GO) platform for fluorescence quenching, a novel, turn-on fluorescent aptasensor for lysozyme (Lys) protein was constructed. The system contains a hairpin probe (HP) and a signal probe... |
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Exonuclease-assisted cascaded recycling amplification for label-free detection of DNA.
Chem. Commun. (Camb.) 48(7) , 1018-20, (2012) The network consisting of three kinds of unlabeled stem-loop DNA molecular beacons (MBs) is activated by target DNA in the presence of exonuclease-III (Exo-III), achieving the concept of exonuclease-assisted cascaded recycling amplification (Exo-CRA) for DNA ... |
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Structural transitions in the transcription elongation complexes of bacterial RNA polymerase during σ-dependent pausing.
Nucleic Acids Res. 40(7) , 3078-91, (2012) A transcription initiation factor, the σ(70) subunit of Escherichia coli RNA polymerase (RNAP) induces transcription pausing through the binding to a promoter-like pause-inducing sequence in the DNA template during transcription elongation. Here, we investiga... |
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A novel exonuclease III aided amplification method for sensitive nucleic acid detection based on single walled carbon nanotube induced quenching.
Chem. Commun. (Camb.) 48(2) , 269-71, (2012) We describe herein a novel exonuclease III aided amplification method based on single walled carbon nanotube quenching (EASQ) for sensitive and convenient nucleic acid detection, which enabled 80-fold decrease of detection limit for HIV1 DNA assay compared wi... |
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Target recycling amplification for sensitive and label-free impedimetric genosensing based on hairpin DNA and graphene/Au nanocomposites.
Chem. Commun. (Camb.) 47(48) , 12798-800, (2011) The presence of exonuclease III leads to direct recycling and reuse of the target DNA, which in turn results in substantial signal amplification for highly sensitive, label-free impedimetric detection of specific DNA sequences.This journal is © The Royal Soci... |
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Two replication fork maintenance pathways fuse inverted repeats to rearrange chromosomes.
Nature 501(7468) , 569-72, (2013) Replication fork maintenance pathways preserve chromosomes, but their faulty application at nonallelic repeats could generate rearrangements causing cancer, genomic disorders and speciation. Potential causal mechanisms are homologous recombination and error-f... |