Ribonuclease S redux.
Rex W Watkins, Ulrich Arnold, Ronald T Raines
文献索引:Chem. Commun. (Camb.) 47(3) , 973-5, (2011)
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摘要
The S-peptide and S-protein components of bovine pancreatic ribonuclease form a noncovalent complex with restored ribonucleolytic activity. Although this archetypal protein-fragment complementation system has been the object of historic work in protein chemistry, intrinsic limitations compromise its utility. Modern methods are shown to overcome those limitations and enable new applications.
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