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镁-吲哚-1乙酰氧甲酯

镁-吲哚-1乙酰氧甲酯用途

Mag-indo-1/AM是Mg2+和Ca2+的细胞渗透荧光指示剂(Ex=340-390 nm,Em=410-490 nm)。Mag-indo-1/AM可用于测定细胞内Mg2+和Ca2+浓度[1][2]。

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镁-吲哚-1乙酰氧甲酯名称

[ CAS 号 ]:
130926-94-2

[ 中文名 ]:
镁-吲哚-1乙酰氧甲酯

[ 英文名 ]:
Mag-indo-1/AM

[英文别名 ]:

Indo 1-AM analog
Mag-Indo 1-AM
Mag-Indo-1AM

镁-吲哚-1乙酰氧甲酯生物活性

[ 描述 ]:

Mag-indo-1/AM是Mg2+和Ca2+的细胞渗透荧光指示剂(Ex=340-390 nm,Em=410-490 nm)。Mag-indo-1/AM可用于测定细胞内Mg2+和Ca2+浓度[1][2]。

[ 相关类别 ]:

研究领域 >> 其他

信号通路 >> 其他 >> 其他

[体外研究]

指南(以下是我们推荐的方案。该方案仅提供指南,应根据您的具体需要进行修改)。细胞内Ca2+的测量[1]：1.在30℃下,用10µM Mag-indo-1/AM培养3 mL细胞悬液15分钟。2.以500 rpm离心细胞1分钟,并在5 mL隔离缓冲液(含有1 mM CaCl2)中再次悬浮。3.向细胞悬浮液中添加1 mM丙磺舒,以防止染料从胞浆中泄漏,并在室温下保存细胞(Mag-indo-1/AM负载细胞),直到使用。4.将装有Mag-indo-1/AM的细胞在室温下培养2小时,在37℃下轻轻摇动1.5小时,离心颗粒,重新悬浮并在室温下储存。6.将一小部分负载细胞放在一个安装在倒置显微镜台上的实验室中。7.测量Mag-indo-1/AM的荧光(Ex=340-390 nm,Em=410和490 nm(指示剂的Ca2+结合和Ca2+游离形式的峰值发射))。整个细胞的荧光通过光子计数系统在线采集。

[参考文献]

[1]. Griffiths EJ. Calcium handling and cell contraction in rat cardiomyocytes depleted of intracellular magnesium. Cardiovasc Res. 2000 Jul;47(1):116-23.

[2]. Morelle B, et al. Measurement of intracellular magnesium concentration in 3T3 fibroblasts with the fluorescent indicator Mag-indo-1. Anal Biochem. 1994 Apr;218(1):170-6.

镁-吲哚-1乙酰氧甲酯物理化学性质

[ 密度 ]:
1.335g/cm3

[ 沸点 ]:
789.9ºC at 760mmHg

[ 分子式 ]:
C₃₀H₃₂N₂O₁₂

[ 分子量 ]:
612.58100

[ 闪点 ]:
431.5ºC

[ 精确质量 ]:
612.19600

[ PSA ]:
176.83000

[ LogP ]:
2.53130

[ 蒸汽压 ]:
6.32E-25mmHg at 25°C

[ 折射率 ]:
1.585

镁-吲哚-1乙酰氧甲酯MSDS

详细MSDS(安全技术说明书)

镁-吲哚-1乙酰氧甲酯安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危险品运输编码 ]:
NONH for all modes of transport

镁-吲哚-1乙酰氧甲酯文献

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