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链霉亲和素

链霉亲和素用途

链霉亲和素(Streptavidin)是一种生物素结合蛋白,是一种多功能的亲和标签。链霉亲和素用于在多种生物学应用中可视化生物素结合分子,包括蛋白质印迹、免疫组织化学和ELISA[1]。

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链霉亲和素名称

[ CAS 号 ]:
9013-20-1

[ 中文名 ]:
链霉亲和素

[ 英文名 ]:
Streptavidin

[中文别名 ]:

链霉青合素

[英文别名 ]:

MFCD00082035
Polystreptavidin

链霉亲和素生物活性

[ 描述 ]:

[ 相关类别 ]:

研究领域 >> 其他

信号通路 >> 其他 >> 其他

[体外研究]

链霉亲和素的非特异性结合水平低于亲和素。链霉亲和素的功效源自其对维生素生物素的极高结合亲和力。链霉亲和素因其独特的生物素高亲和力在分子生物学中得到广泛应用。生物素-链霉亲和素复合物的离解常数(Kd)约为10-15 M[1]。

[参考文献]

[1]. T Sano, wet al. Genetic engineering of streptavidin, a versatile affinity tag. J Chromatogr B Biomed Sci Appl. 1998 Sep 11;715(1):85-91.

链霉亲和素物理化学性质

[ 分子式 ]:
C₁₄H₁₇BrClNO₂S

[ 分子量 ]:
378.71228

[ 外观性状 ]:
lyophilized powder

[ 储存条件 ]:
2-8°C

[ 水溶解性 ]:
H2O: 0.1 mg/mL, clear, colorless

链霉亲和素安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危害码 (欧洲) ]:
Xn,Xi

[ 风险声明 (欧洲) ]:
22-36/37/38-21/22

[ 安全声明 (欧洲) ]:
24/25-36-26-36/37

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

链霉亲和素文献

Cell-permeable probe for identification and imaging of sialidases.

Proc. Natl. Acad. Sci. U. S. A. 110(7) , 2466-71, (2013)

Alkyne-hinged 3-fluorosialyl fluoride (DFSA) containing an alkyne group was shown to be a mechanism-based target-specific irreversible inhibitor of sialidases. The ester-protected analog DFSA (PDFSA) ...

An ultrasensitive streptavidin-functionalized carbon nanotubes platform for chemiluminescent immunoassay.

Anal. Chim. Acta 774 , 85-91, (2013)

An ultrasensitive chemiluminescent (CL) immunoassay system was developed for the detection of tumor marker. This sandwich CL assay method was for the first time designed based on a highly efficient st...

Implementation of a SPR immunosensor for the simultaneous detection of the 22K and 20K hGH isoforms in human serum samples.

Talanta 114 , 268-75, (2013)

We have implemented a Surface Plasmon Resonance (SPR) immunosensor based on a sandwich assay for the simultaneous detection of the two main hGH isoforms, of 22 kDa (22K) and 20 kDa (20K). An oriented-...

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