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Gli1+ Mesenchymal Stromal Cells Are a Key Driver of Bone Marrow Fibrosis and an Important Cellular Therapeutic Target
10.1016/j.stem.2017.03.008 2017-04-27 Bone marrow fibrosis (BMF) develops in various hematological and non-hematological conditions and is a central pathological feature of myelofibrosis. Effective cell-targeted therapeutics are needed, but the cellular origin of BMF remains elusive. Here, we sho... |
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Single-Cell RNA-Seq Analysis Maps Development of Human Germline Cells and Gonadal Niche Interactions
10.1016/j.stem.2017.03.007 2017-04-27 Human fetal germ cells (FGCs) are precursors to sperm and eggs and are crucial for maintenance of the species. However, the developmental trajectories and heterogeneity of human FGCs remain largely unknown. Here we performed single-cell RNA-seq analysis of ov... |
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Single-Cell RNA-Seq Analysis Maps Development of Human Germline Cells and Gonadal Niche Interactions
10.1016/j.stem.2017.03.007 2017-04-27 Li et al. interrogate the transcriptomes of over 2,000 fetal germ cells (FGCs) and their gonadal niche cells from male and female human embryos using single-cell RNA-seq analysis. They provide insights into the developmental trajectories and heterogeneity in ... |
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Gli1+ Mesenchymal Stromal Cells Are a Key Driver of Bone Marrow Fibrosis and an Important Cellular Therapeutic Target
10.1016/j.stem.2017.03.008 2017-04-27 Schneider and colleagues show that Gli1+ bone marrow mesenchymal stromal cells are an important source of fibrotic cells during bone marrow fibrosis and that targeting of Gli proteins with GANT61 holds promise for amelioration of this disease. |
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An FAK-YAP-mTOR Signaling Axis Regulates Stem Cell-Based Tissue Renewal in Mice
10.1016/j.stem.2017.03.023 2017-04-27 Klein and colleagues show, using the mouse incisor as a model, that the transcriptional cofactors YAP and TAZ, components of the Hippo pathway, regulate stem cell-based tissue renewal by controlling the proliferation and differentiation of transit-amplifying ... |
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Induced Pluripotent Stem Cell Differentiation Enables Functional Validation of GWAS Variants in Metabolic Disease
10.1016/j.stem.2017.01.010 2017-04-06 As part of the NHLBI NextGen consortium, Warren et al. show that analyzing the cellular pathophysiology of cells differentiated from multiple lines of an iPSC library is a promising complementary approach in the functional analysis of GWAS variants. |
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A Drug Screen using Human iPSC-Derived Hepatocyte-like Cells Reveals Cardiac Glycosides as a Potential Treatment for Hypercholesterolemia
10.1016/j.stem.2017.01.011 2017-04-06 Cayo et al. of the NHLBI NextGen consortium use hepatocytes from familial hypercholesterolemia iPSCs in a drug screen that highlights cardiac glycosides as a candidate treatment acting to reduce apoB levels via proteolytic turnover and as a result lower LDL-C... |
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Of Mice and Men: Species-Specific Organoid Models of Neocortical Malformation
10.1016/j.stem.2017.03.005 2017-04-06 Cellular changes underlying malformations of human cortical development may be difficult to identify with traditional mouse models. Two recent Cell Stem Cell papers, Li et al. (2017) and Bershteyn et al. (2017), use human cerebral organoids to identify specif... |
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DEAD-Box RNA Binding Protein DDX5: Not a Black-Box during Reprogramming
10.1016/j.stem.2017.03.006 2017-04-06 The role of RNA binding proteins (RBPs) during nuclear reprogramming is poorly characterized. In this issue of Cell Stem Cell, Li et al. (2017) show that DEAD-box RBP DDX5 acts as a reprogramming roadblock and give important mechanistic insights into the esta... |
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Large-Scale Profiling Reveals the Influence of Genetic Variation on Gene Expression in Human Induced Pluripotent Stem Cells
10.1016/j.stem.2017.03.009 2017-04-06 Working as part of the NextGen consortium, DeBoever et al. use whole-genome and RNA sequencing to map expression quantitative trait loci in a set of 215 human induced pluripotent stem cell lines. These genotype-expression associations provide a foundation for... |