Syntheses of labeled vitamers of folic acid to be used as internal standards in stable isotope dilution assays

A Freisleben, P Schieberle…

Index: Freisleben, Achim; Schieberle, Peter; Rychlik, Michael Journal of Agricultural and Food Chemistry, 2002 , vol. 50, # 17 p. 4760 - 4768

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Citation Number: 47

Abstract

[2H4] Folic acid was synthesized by deuterating p-aminobenzoic acid, which was then coupled to glutamic acid and 6-formylpterin. Using [2H4] folic acid as starting component enabled the preparation of labeled vitamers tetrahydrofolate, 5-formyltetrahydrofolate, 5- methyltetrahydrofolate, and 10-formylfolate which were characterized by electrospray mass spectrometry and collision-induced dissociation. The mass spectrometric studies ...