The Catalytic Potential of Coptis japonica NCS2 Revealed–Development and Utilisation of a Fluorescamine??Based Assay

T Pesnot, MC Gershater, JM Ward…

Index: Pesnot, Thomas; Gershater, Markus C.; Ward, John M.; Hailes, Helen C. Advanced Synthesis and Catalysis, 2012 , vol. 354, # 16 p. 2997 - 3008

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Citation Number: 23

Abstract

Abstract The versatility and potential of a norcoclaurine synthase (NCS) from Coptis japonica NCS2 has been investigated, together with the development and application of a novel fluorescence-based high-throughput assay using nearly forty amines/aldehydes. The stereocontrol exerted by CjNCS2 on selected non-natural substrates has been determined, where the tetrahydroisoquinolines (THIAs) were formed as the (1S)-isomer in> 95% ee, as ...