Antonie Van Leeuwenhoek 2015-09-01

Hydrogenophaga luteola sp. nov. isolated from reed pond water.

Juan Du, Jung-Eun Yang, Hina Singh, Shahina Akter, KyungHwa Won, Chang Shik Yin, Feng-Xie Jin, Tae-Hoo Yi

Index: Antonie van Leeuwenhoek 108 , 695-701, (2015)

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Abstract

A yellowish colored, Gram-staining negative, strictly aerobic, motile, rod-shaped bacterium, designated THG-SQE7(T), was isolated from reed pond water in Shangqiu, PR China. Comparative 16S rRNA gene sequence analysis indicated that strain THG-SQE7(T) is most closely related to Hydrogenophaga pseudoflava ATCC 33668(T) (98.4 %), followed by Hydrogenophaga bisanensis K102(T) (97.6 %) and Hydrogenophaga flava CCUG 1658(T) (97.6 %). DNA-DNA hybridization showed 53.5, 36.0 and 22.5 % DNA re-association with H. pseudoflava KCTC 2348(T), H. bisanensis KCTC 12980(T) and H. flava KCTC 1648(T), respectively. Chemotaxonomic data revealed that strain THG-SQE7(T) possesses ubiquinone-8 as the only isoprenoid quinone, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C16:0 and C18:1 ω7c as the major fatty acids. The major polar lipids were found to be phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was determined to be 63.7 mol%. These data corroborated the affiliation of strain THG-SQE7(T) to the genus Hydrogenophaga. Thus, the isolate represents a novel species, for which the name Hydrogenophaga luteola sp. nov. is proposed, with THG-SQE7(T) as the type strain (=KCTC 42501(T) = CCTCC AB 2014314(T) = JCM 30433(T)).