Plant Science 2014-10-01

Plant regeneration and biochemical accumulation of hydroxybenzoic and hydroxycinnamic acid derivatives in Hypoxis hemerocallidea organ and callus cultures.

Mack Moyo, Stephen O Amoo, Adeyemi O Aremu, Jiří Gruz, Michaela Subrtová, Karel Doležal, Johannes Van Staden

Index: Plant Sci. 227 , 157-64, (2014)

Full Text: HTML

Abstract

Micropropagation of Hypoxis hemerocallidea Fisch. and C.A. Mey was used as a model system to study the influence of cytokinins (CKs) on plant regeneration and biochemical accumulation of hydroxybenzoic and hydroxycinnamic acid derivatives in organ and callus cultures and their antioxidant activity. Fourteen free phenolic acids were detected using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) while antioxidant activity was evaluated using oxygen radical absorbance capacity (ORAC) and 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activity. Cytokinins had a significant effect on the biochemical accumulation of hydroxybenzoic and hydroxycinnamic acid derivatives in H. hemerocallidea organ cultures. In particular, meta-topolin-treated organ cultures produced high concentrations of gallic, protocatechuic, gentisic, p-hydroxybenzoic, m-hydroxybenzoic, salicylic, chlorogenic and trans-cinnamic acids. The isoprenoid CK, N(6)-(2-isopentenyl)-adenine significantly increased the accumulation of hydroxycinnamic acid derivatives, namely, caffeic, p-coumaric, sinapic and ferulic acids. Cytokinin-treated organ cultures exhibited a significant increase in antioxidant activity, particularly in the ORAC model. In callus cultures, CKs decreased the concentrations of hydroxycinnamic acid derivatives and antioxidant activity when compared to the control. Overall, both CK type and concentration had a significant effect on plant regeneration, callus proliferation, biochemical accumulation of free phenolic acids and antioxidant activity of the resultant extracts. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Related Compounds

Structure Name/CAS No. Articles
Formic Acid Structure Formic Acid
CAS:64-18-6
Fluorescein sodium Structure Fluorescein sodium
CAS:518-47-8
Glycine Structure Glycine
CAS:56-40-6
Methanol Structure Methanol
CAS:67-56-1
deuterium Structure deuterium
CAS:7782-39-0
Nicotinic acid Structure Nicotinic acid
CAS:59-67-6
Vitamin E Structure Vitamin E
CAS:10191-41-0
Pyridoxine Structure Pyridoxine
CAS:65-23-6
Inositol Structure Inositol
CAS:87-89-8
DL-alpha-Tocopherol Structure DL-alpha-Tocopherol
CAS:59-02-9