Photodiagnosis and Photodynamic Therapy 2012-06-01

Evaluation of collagen alteration after topical photodynamic therapy (PDT) using second harmonic generation (SHG) microscopy--in vivo study in a mouse model.

Ting Lv, Zu-Fang Huang, Hong-Wei Wang, Ju-Qiang Lin, Guan-Nan Chen, Xi-Wen Chen, Rong Chen, Zheng Huang, Xiu-Li Wang

Index: Photodiagnosis Photodyn. Ther. 9(2) , 164-9, (2012)

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Abstract

Topical photodynamic therapy (PDT) mediated by 5-aminolevulinic acid (ALA) has been used for the treatment of age-related skin lesions for therapeutic or cosmetic purposes. The modulation of collagen component and structure might play a significant role in influencing treatment outcomes of PDT. In this study, the effect of multi-session low dose ALA PDT on skin rejuvenation was examined in a hairless mouse model. Changes in collagen and skin texture were investigated by histological examination and in vivo second harmonic generation (SHG) microscopy. Results indicated that multi-session PDT could improve the collagen density and arrangement of skin tissue. SHG microscopy combined with quantitative collagen analysis could provide a useful tool for the evaluation of collagen alteration induced by ALA PDT.Copyright © 2011 Elsevier B.V. All rights reserved.