Journal of Neurochemistry 1988-08-01

Formation of gamma-amino-beta-hydroxybutyric acid from 2-hydroxyputrescine in rat brain.

T Noto, T Hasegawa, J Nakao, H Kamimura, H Harada, T Nakajima

Index: J. Neurochem. 51(2) , 548-51, (1988)

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Abstract

gamma-Amino-beta-[3H]hydroxybutyric acid ([3H]-GABOB) was formed in rat brain from 2-[3H]-hydroxyputrescine that had been chemically synthesized from 2-oxoputrescine and [3H]sodium borohydride. After the injection of 2-[3H]hydroxyputrescine into the lateral ventricle of a rat brain, the rat was killed and then the brain was removed. [3H]GABOB in the brain was identified by a combination of ion-exchange chromatography, high-voltage paper electrophoresis, and recrystallization of the radioactive compound with authentic GABOB.