210345-02-1

210345-02-1 structure

Name: Ac-Leu-Glu-Thr-Asp-AFC
Chemical Name: 4-[(2-acetamido-4-methylpentanoyl)amino]-5-[[1-[[3-carboxy-1-oxo-1-[[2-oxo-4-(trifluoromethyl)chromen-7-yl]amino]propan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-5-oxopentanoic acid
CAS Number: 210345-02-1
Molecular Formula: C₃₁H₃₈F₃N₅O₁₂
Molecular Weight: 729.655
Catalog: Research Areas Cancer
Create Date: 2017-04-07 20:41:32
Modify Date: 2025-08-27 12:56:07
Ac-LETD-AFC is a caspase-8 fluorogenic substrate. Ac-LETD-AFC can measure caspase-8 fluorogenic activity and can be used for the research of cancer cell apoptosis and oxidative stress metabolism[1].

Name	4-[(2-acetamido-4-methylpentanoyl)amino]-5-[[1-[[3-carboxy-1-oxo-1-[[2-oxo-4-(trifluoromethyl)chromen-7-yl]amino]propan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-5-oxopentanoic acid
Synonyms	Ac-Leu-Glu-Thr-Asp-7-Amino-4-trifluoromethylcoumarin AFC157 Ac-LGlutD-AFC Ac-Leu-Glu-Thr-Asp-AFC L-α-Asparagine, N-acetyl-L-leucyl-L-α-glutamyl-L-threonyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]- N-Acetyl-L-leucyl-L-α-glutamyl-L-threonyl-N-[2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl]-L-α-asparagine

Description	Ac-LETD-AFC is a caspase-8 fluorogenic substrate. Ac-LETD-AFC can measure caspase-8 fluorogenic activity and can be used for the research of cancer cell apoptosis and oxidative stress metabolism[1].
Related Catalog	Research Areas >> Cancer Signaling Pathways >> Others >> Others Research Areas >> Metabolic Disease
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Caspase activity assay[1]: 1. Incubate the cells according to your normal protocol. 2. Wash cells once with PBS and centrifugate. 3. Resuspend cells in PBS at a concentration of 1× 107 cells/mL. 4. Prepare the standard reaction buffer which includ 100 μM caspase-8 substrate AC-LETD-AFC. Prepare correspounding standard reaction buffer according to your protocol. 5. Add 15 ul of the cells suspension to a microplate, and mixed with the appropriate peptide substrate dissolved in a standard reaction buffer. 6. Measure substrate cleavage with a microplate reader with excitation wavelength of 360 nm and emission at 460 nm.