466-61-5
466-61-5 structure
- Name: Lycopodine
- Chemical Name: lycopodine
- CAS Number: 466-61-5
- Molecular Formula: C16H25NO
- Molecular Weight: 247.37600
- Catalog: Signaling Pathways Apoptosis Caspase
- Create Date: 2018-08-05 09:32:26
- Modify Date: 2024-01-02 22:24:07
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Lycopodine, a pharmacologically important bioactive component derived from Lycopodium clavatumspores, triggers apoptosis by modulating 5-lipoxygenase, and depolarizing mitochondrial membrane potential in refractory prostate cancer cells without modulating p53 activity[1]. Lycopodine inhibits proliferation of HeLa cells through induction of apoptosis via caspase-3 activation[2].
| Name | lycopodine |
|---|---|
| Synonyms | (15R)-15-Methyllycopodan-5-one |
| Description | Lycopodine, a pharmacologically important bioactive component derived from Lycopodium clavatumspores, triggers apoptosis by modulating 5-lipoxygenase, and depolarizing mitochondrial membrane potential in refractory prostate cancer cells without modulating p53 activity[1]. Lycopodine inhibits proliferation of HeLa cells through induction of apoptosis via caspase-3 activation[2]. |
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| Related Catalog | |
| Target |
Caspase-3 |
| In Vitro | Lycopodine (5.22-78.3 μg/mL; 12 hours) has 50% viability at 57.62±0.086 μg/mL and 51.46±1.43 μg/mL for PC3 and LnCaP, respectively[1]. Treated with Lycopodine (74-222 mM; 12 hours), the apoptotic index is with respect to the gradual increase in doses for the PC3 and LnCaP cells[1]. Lycopodine (74-222 mM; 12 hours) induces cell cycle arrest at G0/G1 phase in PC3 and LnCaP cells[1]. Lycopodine (0-200 µg/mL; 48 hours) shows cytotoxicity to HeLa cells in a dose and time dependent manner. However, Lycopodine shows minimal cytotoxic effects in normal peripheral blood mononuclear cells (PBMC) even at the highest dose (200 µg/mL)[2]. Lycopodine (100, 200 µg/mL; 24 hours) increases level of Bax and decreases the mitochondrial cytochrome c. This is followed by an increase in expression of cytochrome c in cytosolic fraction. Lycopodine also cleaves the caspase-3 in the total cell lysate, while the expression of Bcl-2 is down regulated[2]. Cell Viability Assay[1] Cell Line: PC3 and LnCaP cells Concentration: 5.22-78.3 μg/mL Incubation Time: 12 hours Result: Reached 50% viability at 57.62±0.086 μg/mL and 51.46±1.43 μg/mL for PC3 and LnCaP, respectively. Apoptosis Analysis[1] Cell Line: PC3 and LnCaP cells Concentration: 74, 148, 222 mM Incubation Time: 12 hours Result: The apoptotic index was with respect to the gradual increase in doses. Cell Cycle Analysis[1] Cell Line: PC3 and LnCaP cells Concentration: 74, 148, 222 mM Incubation Time: 12 hours Result: Arrested growth of the cells at G0/G1 phase in the case of PC3 and LnCaP cells. Cell Cytotoxicity Assay[2] Cell Line: Hela cells and PBMC Concentration: 0-200 µg/mL Incubation Time: 48 hours Result: A linear increase of the cytotoxicity was along with the increase of time of treatment and also of the dose. Western Blot Analysis[2] Cell Line: HeLa cells Concentration: 100, 200 µg/mL Incubation Time: 24 hours Result: Increased level of Bax and decreased the mitochondrial cytochrome c. |
| References |
| Density | 1.106g/cm3 |
|---|---|
| Boiling Point | 373.464ºC at 760 mmHg |
| Molecular Formula | C16H25NO |
| Molecular Weight | 247.37600 |
| Flash Point | 137.245ºC |
| Exact Mass | 247.19400 |
| PSA | 20.31000 |
| LogP | 2.94810 |
| Vapour Pressure | 0mmHg at 25°C |
| Index of Refraction | 1.556 |
| HS Code | 2933990090 |
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| Precursor 4 | |
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| DownStream 0 | |
| HS Code | 2933990090 |
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| Summary | 2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0% |