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119171-73-2

119171-73-2 structure
119171-73-2 structure
  • Name: Ampliflu Red
  • Chemical Name: ADHP  [10-Acetyl-3,7-dihydroxyphenoxazine]
  • CAS Number: 119171-73-2
  • Molecular Formula: C14H11NO4
  • Molecular Weight: 257.241
  • Catalog: Dye Reagents
  • Create Date: 2018-12-29 09:05:04
  • Modify Date: 2024-01-05 09:14:46
  • ADHP is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm).

Name ADHP  [10-Acetyl-3,7-dihydroxyphenoxazine]
Synonyms 10-Acetyl-10H-phenoxazine-3,7-diol
1-(3,7-Dihydroxy-10H-phenoxazin-10-yl)ethanone
1-(3,7-dihydroxyphenoxazin-10-yl)ethanone
MFCD00467861
N-acetyl-3,7-dihydroxyphenoxazine
10H-Phenoxazine-3,7-diol, 10-acetyl-
Ampliflu™ Red
Amplex red
Ethanone, 1-(3,7-dihydroxy-10H-phenoxazin-10-yl)-
10-Acetyl-3,7-dihydroxyphenoxazine
ADHP
Description ADHP is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm).
Related Catalog
In Vitro To obtain the parameters Km and kcat for Compound I, two independent methods are used. Initially, the oxidation of ADHP using the injector functionality built-in to the fluorescence plate reader is studied. The auto-injector dispenses the H2O2 to initiate the reaction, as a means of generating a set of progress curves. Analysis for MPO-mediated oxidation of ADHP gives a Km of 31±4 μM and the kcat of 186± 6 s−1.The kobs also increases over the experimental range of ADHP concentrations from 1 to 80 μM and for the converse experiment holding substrate constant over 3 to 45 nM MPO. The apparent second order rate constant obtain from the slope of kobs against ADHP concentration Kappon is 2.1±0.2 mM/s[1].
Kinase Assay ADHP, 4-ABAH, 2-ABAH, 4-BAH, 4-FBAH, 4-NBAH, 4-TFMBAH, 3-DMABAH, NaN3 and isoniazid are dissolved in DMSO and subsequently diluted into assay buffer. The final concentration of DMSO in the reaction is less than 0.5 % (v/v), which does not affect fluorescence of the oxidized ADHP product 7-hydroxyl-3H-phenoxazin-3-one (resorufin). Reactions of ADHP (20 μM) are incubated with MPO (2.8 nm) in assay buffer and initiated by the addition of 1/10th volume H2O2 from a serial dilution basin. To determine the effect that the simplest benzoic acid hydrazide inhibitor or its analog 4-TFMBAH has on the heme catalytic ability of MPO, MPO (1.2 μM) is incubated for 10 min with different concentrations of BAH inhibitor (0, 0.025, 0.25, 2.5, 12.5 and 25 mM) with ADHP (40 μM) and timing of the reaction is measured following addition of H2O2 (20 μM) ADHP. All reactions are measured in assay buffer at room temperature. Samples of 20 μL are added to non-reducing sample loading buffers, and then loaded without prior heating and resolved by 4-15% gradient SDS-polyacrylamide gel electrophoresis[1].
References

[1]. Jiansheng Huang, et al. Ordered Cleavage of Myeloperoxidase Ester Bonds Releases Active site Heme Leading to Inactivation of Myeloperoxidase by Benzoic Acid Hydrazide Analogs. Arch Biochem Biophys. 2014 Apr 15; 548: 74–85.

Density 1.5±0.1 g/cm3
Boiling Point 618.6±55.0 °C at 760 mmHg
Molecular Formula C14H11NO4
Molecular Weight 257.241
Flash Point 327.9±31.5 °C
Exact Mass 257.068817
PSA 70.00000
LogP 0.89
Vapour Pressure 0.0±1.9 mmHg at 25°C
Index of Refraction 1.689
Symbol GHS07
GHS07
Signal Word Warning
Hazard Statements H302
Personal Protective Equipment dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes Xn: Harmful;
Risk Phrases R22
RIDADR NONH for all modes of transport
HS Code 2934999090
HS Code 2934999090
Summary 2934999090. other heterocyclic compounds. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%