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166090-74-0

166090-74-0 structure

166090-74-0 structure

Name: Amyloid β-Protein (1-42) (mouse, rat)
Chemical Name: Amyloid beta-peptide (1-42) (rat)
CAS Number: 166090-74-0
Molecular Formula: C₁₉₉H₃₀₇N₅₃O₅₉S
Molecular Weight: 4418.017
Catalog: Peptides
Create Date: 2018-04-09 08:00:00
Modify Date: 2024-01-02 12:46:59
β-Amyloid (1-42), rat is a 42-aa peptide, shows cytotoxic effect on acute hippocampal slices, and used in the research of Alzheimer's disease.

Name	Amyloid beta-peptide (1-42) (rat)
Synonyms	bap 1-42 asp-ala-glu-phe-arg-his-asp-ser-gly-tyr-glu-val-his-his-gln-lys-leu-val-phe-phe-ala-glu-asp-val-gly-ser-asn-lys-gly-ala-ile-ile-gly-leu-met-val-gly-gly-val-val-ile-ala predominant amyloid b-protein fragment beta-amyloid peptide (1-40), mouse, rat asp-ala-glu-phe-gly-his-asp-ser-gly-phe-glu-val-arg-his-gln-lys-leu-val-phe-phe-ala-glu-asp-val-gly-ser-asn-lys-gly-ala-ile-ile-gly-leu-met-val-gly-gly-val-val-ile-ala beta-amyloid peptide (1-40), rat beta-amyloid, sodium salt beta-amyloid peptide (1-42), rat β-Amyloid (1-42), rat

Description	β-Amyloid (1-42), rat is a 42-aa peptide, shows cytotoxic effect on acute hippocampal slices, and used in the research of Alzheimer's disease.
Related Catalog	Signaling Pathways >> Neuronal Signaling >> Amyloid-β Peptides Research Areas >> Neurological Disease
Target	Amyloid-β[1]
In Vitro	β-Amyloid (1-42), rat shows cytotoxic effect on the hippocampal slices at 20 μM[1]. β-Amyloid (1-42), rat causes morphological changes in NGF-induced PC12 cells, induces formed cell processes to retract in differentiated cells and affect the expression of exons 2/3 in both undifferentiated and differentiated cells[2].
Cell Assay	After treating the hippocampal slices with 20 μM β-Amyloid (1-42) for 4 h, supernatant is replaced by fresh H-ASCF/3 (0.9 mL/chamber) to which 0.1 mL MTT stock solution (5 mg/mL H-ACSF/3) is added (MTT final concentration: 0.5 mg/mL). The chamber is left to rest for 15 min without carboxygenation. To stop further reduction of MTT, the medium (H-ACSF/3) is removed. The slices are transferred into 96-well plate, then pure DMSO (100 μL/slice/well) is added for dissolving formazane from the slices. (30 min in a 96-well plate). Then 70 μL DMSO solution from each slice (well) is transferred into another 96-well plate. The optical density (OD) of the dissolved formazane is measured at 550 and 620 nm[1].
References	[1]. Mozes E, et al. A novel method for the rapid determination of beta-amyloid toxicity on acute hippocampal slices using MTT and LDH assays. Brain Res Bull. 2012 Apr 10;87(6):521-5. [2]. Lagunes T, et al. Abeta(1-42) induces abnormal alternative splicing of tau exons 2/3 in NGF-induced PC12 cells. An Acad Bras Cienc. 2014 Dec;86(4):1927-34.

Molecular Formula	C₁₉₉H₃₀₇N₅₃O₅₉S
Molecular Weight	4418.017
Exact Mass	4417.244