910661-33-5
910661-33-5 structure
- Name: EC-17 disodium salt
- Chemical Name: 5-{[(4-Chlorophenyl)carbamoyl]amino}-N-cyclopropyl-2-(3,4-dihydro-2(1H)-isoquinolinyl)benzamide
- CAS Number: 910661-33-5
- Molecular Formula: C42H36N10NaO10S+
- Molecular Weight: 895.85
- Catalog: Research Areas Cancer
- Create Date: 2018-07-12 21:42:41
- Modify Date: 2024-01-07 11:51:34
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EC-17 (disodium salt) is a folate receptor alpha (FRα) targeting contrast agent with fluorescent properties in the visible light spectrum. The peak excitation and emission wavelengths of EC-17 are 470/520 nm.
| Name | 5-{[(4-Chlorophenyl)carbamoyl]amino}-N-cyclopropyl-2-(3,4-dihydro-2(1H)-isoquinolinyl)benzamide |
|---|---|
| Synonyms |
5-{[(4-Chlorophenyl)carbamoyl]amino}-N-cyclopropyl-2-(3,4-dihydro-2(1H)-isoquinolinyl)benzamide
Benzamide, 5-[[[(4-chlorophenyl)amino]carbonyl]amino]-N-cyclopropyl-2-(3,4-dihydro-2(1H)-isoquinolinyl)- 5-[[[[2-[[(4S)-4-[[4-[[(2-Amino-1,4-dihydro-4-oxo-6-pteridinyl)methyl]amino]benzoyl]amino]-4-carboxy-1-oxobutyl]amino]ethyl]amino]thioxomethyl]amino]-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid sodium salt |
| Description | EC-17 (disodium salt) is a folate receptor alpha (FRα) targeting contrast agent with fluorescent properties in the visible light spectrum. The peak excitation and emission wavelengths of EC-17 are 470/520 nm. |
|---|---|
| Related Catalog | |
| Target |
Folate receptor alpha[1] |
| In Vitro | EC-17 contains the fluorescein fluorochrome and has a spectral wavelength of 490–530 nm. EC-17 is synthesized by a folate (vitamin B9) and fluorescein isothiocyanate (FITC) conjugated through an ethylenediamine spacer to produce folate-FITC, with a molecular weight of 917 kDa. FITC is a derivative of fluorescein functionalized with an isothiocyanate reactive group. The folate-FITC conjugate forms a negatively charged fluorescent molecule that specifically targets cell-surface FRα and is subsequently internalized into the cytoplasm. The signal-to-background ratio (SBR) of EC-17 for HeLa cells range from 0.97 to 7.32 depending on the molarity and concentration of cancer cells[1]. |
| In Vivo | The mean fluorescence signal from the animals injected with EC-17 to be 42,234 ± 12,234 au[1]. Fluorescence microscopy for folate-FITC shows a strong signal in all malignant tumors with FR-α expression and no signal in FR-α–negative malignant or benign lesions[2]. |
| Cell Assay | KB, HeLa, and TC1 cells are plated on a cell culture treated 6-well plate and incubated for 16 hours. Once confluent, EC-17 is added cells. The cells are incubated and sealed in a light-protected environment for 45 minutes. Cells are then washed 3 times with PBS and plated and underwent fluorescence microscopy[1]. |
| Animal Admin | Mice[1] Mice are injected subcutaneously in the flank with 1.2×106 TC1 cells (C57BL/6 mice), 1.0×106 HeLa cells (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ mice), or 1.0×106 KB cells (NOD.Cg-Prkdcscid, Il2rgtm1Wjl/SzJ mice). Once tumor volume reached approximately 300 mm3 half of the mice are injected with 0.1 mg/kg of EC-17 and the other half with 0.1 mg/kg of OTL38 via tail vein. Three hours later, the fluorescence of tumors is measured using Flocam[1]. |
| References |
| Density | 1.4±0.1 g/cm3 |
|---|---|
| Boiling Point | 591.3±50.0 °C at 760 mmHg |
| Molecular Formula | C42H36N10NaO10S+ |
| Molecular Weight | 895.85 |
| Flash Point | 311.4±30.1 °C |
| LogP | 4.72 |
| Vapour Pressure | 0.0±1.7 mmHg at 25°C |
| Index of Refraction | 1.696 |