| In Vitro |
HDAC8 degradation induced by SZUH280 (5 μM; 20 h) is mediated by the CRBN E3 ubiquitin ligase[1]. SZUH280 (0.1-10 μM; 20 h) can regulate the oncogenic protein expression and suppress cancer metastasis, potentially improving the efficacy of chemotherapy in various types of cancers[1]. SZUH280 (0-20 μM; 72 h) inhibits A549 cell proliferation in a concentration-dependent manner and shows stronger antiproliferative effect with irradiation[1]. SZUH280 (0-20 μM; 72 h) induces apoptosis and arrests cell cycle at G2/M phase in A549 cells[1]. SZUH280 (5 μM; 24 h) hampers DNA damage repair in cancer cells when in combination with irradiation[1]. Western Blot Analysis[1] Cell Line: A549, HCT116, HeLa and MDA-MB-231 cells Concentration: 0.1, 0.3, 1, 3 and 10 μM Incubation Time: 20 h Result: Efficiently induced HDAC8 degradation in a concentration-dependent manner in A549, HCT116 and HeLa cells. Reduced the IKZF1 protein levels to a lesser extent at 10 μM in A549 cells. Decreased PGM1 expression during glucose deprivation conditions in A549 cells. Decreased SMAD3 and HDAC8 protein levels in MDA-MB-231 cells. Cell Proliferation Assay[1] Cell Line: A549 cells Concentration: 2.5, 5, 10 and 20 μM Incubation Time: 72 h Result: Effectively inhibited cell proliferation in a concentration-dependent manner with an IC50 of 9.55 μM. Co-treatment with irradiation exhibited an even stronger antiproliferative effect (with an IC50 value of about 6.04 μM). Apoptosis Analysis[1] Cell Line: A549 cells Concentration: 1.25, 2.5, 5, 10 and 20 μM Incubation Time: 72 h Result: Effectively induced apoptosis in a dose-dependent manner. Cell Cycle Analysis[1] Cell Line: A549 cells Concentration: 1.25, 2.5, 5, 10 and 20 μM Incubation Time: 72 h Result: Induced cell cycle arrest at the G2/M phase.
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