DC Chemicals Limited
China
Product Name: 4μ8C
Price: $350.0/100mg $600.0/250mg $1200.0/1g
Purity: 98.0%
Stocking Period: 3 Day
Contact: Tony Cao

14003-96-4

14003-96-4 structure

14003-96-4 structure

Name: 4μ8C
Chemical Name: 7-hydroxy-4-methyl-2-oxochromene-8-carbaldehyde
CAS Number: 14003-96-4
Molecular Formula: C₁₁H₈O₄
Molecular Weight: 204.17900
Catalog: Signaling Pathways Cell Cycle/DNA Damage IRE1
Create Date: 2019-01-02 14:12:12
Modify Date: 2024-01-20 20:47:43
4μ8C (IRE1 Inhibitor III) is a small-molecule inhibitor of IRE1α.

Name	7-hydroxy-4-methyl-2-oxochromene-8-carbaldehyde
Synonyms	7-Hydroxy-4-methyl-2-oxo-2H-1-benzopyran-8-carboxaldehyde 7-hydroxy-4-methyl-2-oxo-2H-chromen-8-carbaldehyde 7-hydroxy-4-methyl-2-oxo-2H-chromene-8-carbaldehyde 2H-1-Benzopyran-8-carboxaldehyde,7-hydroxy-4-methyl-2-oxo 8-formyl-7-hydroxy-4-methyl-coumarin 8-formyl-4-methyl-7-hydroxycoumarin 8-formyl-7-hydroxy-4-methyl-2H-<1>-benzopyran-2-one 4μ8C 8-Formyl-7-hydroxy-4-methylcoumarin

Description	4μ8C (IRE1 Inhibitor III) is a small-molecule inhibitor of IRE1α.
Related Catalog	Signaling Pathways >> Cell Cycle/DNA Damage >> IRE1 Research Areas >> Metabolic Disease
In Vitro	When applies to the media of ER stressed cultured cells, 4μ8C inhibits Xbp1 splicing in a concentration-dependent manner. 4μ8C dissociates slowly from IRE1, but ishout of inhibitor leads to rapid recovery of Xbp1 splicing in cells[1].The IRE1 endoribonuclease inhibitor 4μ8c prevents the splicing of the XBP1 mRNA in response to ER stress caused by mutant proinsulin production[2]. The inositol-requiring enzyme 1α (IRE1α) is a serine-threonine kinase that plays crucial roles in activating the unfolded protein response. 4μ8C treatment dramatically inhibits IL-4 production by CD4+ T cells under Th0 conditions because both the IL-4 levels in the culture supernatant and the percentage of IL-4 positive cells are reduced by 4μ8C treatment. In addition, both IL-5 and IL-13 production are significantly reduced upon treatment with 4μ8C[3].
In Vivo	4μ8C reverses the ER stress-dependent loss of several known RIDD targets, with an EC50 of approximately 4 μM, approximating that of inhibition of XBP1 target gene activation[1].
Cell Assay	INS-1 (Insulin 2 C96Y-GFP) cells (clone #4S2) cells are either left untreated or treated with 2 μg/mL doxycycline, 2 μg/mL doxycycline and 5 μM 4μ8C or 5 μM 4μ8C alone. After 48 h 50,000 cells/100 μL of media from each treatment well are seeded into a 96-well plate in duplicates. The CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay MTS is performed. The absorbance at 490 nm is then measured with a plate reader.[2].
References	[1]. Cross BC, et al. The molecular basis for selective inhibition of unconventional mRNA splicing by an IRE1-binding small molecule. Proc Natl Acad Sci U S A. 2012 Apr 10;109(15):E869-78. [2]. Zhang L, et al. IRE1 inhibition perturbs the unfolded protein response in a pancreatic β-cell line expressing mutant proinsulin, but does not sensitize the cells to apoptosis. BMC Cell Biol. 2014 Jul 10;15:29. [3]. Kemp K, et al. The serine-threonine kinase inositol-requiring enzyme 1α (IRE1α) promotes IL-4 production in T helper cells. J Biol Chem. 2013 Nov 15;288(46):33272-82.

Density	1.406±0.06 g/cm3 (20 ºC 760 Torr)
Melting Point	189-190 ºC (ethanol )
Molecular Formula	C₁₁H₈O₄
Molecular Weight	204.17900
Exact Mass	204.04200
PSA	67.51000
LogP	1.61950
Storage condition	-20℃
Water Solubility	Very slightly soluble (0.59 g/L) (25 ºC)

Symbol	GHS07
Signal Word	Warning
Hazard Statements	H302
RIDADR	NONH for all modes of transport
HS Code	2932209090

Precursor 9
90-33-5 100-97-0 3993-57-5 64-19-7
1402244-59-0 108-46-3 86290-39-3 141-97-9
68-12-2
DownStream 2
4115-76-8 2107-77-9

HS Code	2932209090
Summary	2932209090. other lactones. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%