150651-39-1
150651-39-1 structure
- Name: iFSP1
- Chemical Name: 1-amino-3-(p-tolyl)benzo[4,5]imidazo[1,2-a]pyridine-2,4-dicarbonitrile
- CAS Number: 150651-39-1
- Molecular Formula: C20H13N5
- Molecular Weight: 323.35100
- Catalog: Signaling Pathways Apoptosis Ferroptosis
- Create Date: 2016-11-07 14:06:20
- Modify Date: 2025-08-25 17:11:53
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iFSP1 is a potent, selective and glutathione-independent inhibitor of ferroptosis suppressor protein 1 (FSP1) (AIFM2) with an EC50 of 103 nM. iFSP1 selectively induces ferroptosis in GPX4-knockout cells which overexpressed FSP1. iFSP1 is able to sensitize a variety of human cancer cell lines to the ferroptosis inducer, such as (1S,3R)-RSL3 (HY-100218A)[1].
| Name | 1-amino-3-(p-tolyl)benzo[4,5]imidazo[1,2-a]pyridine-2,4-dicarbonitrile |
|---|---|
| Synonyms | MFCD01572665 |
| Description | iFSP1 is a potent, selective and glutathione-independent inhibitor of ferroptosis suppressor protein 1 (FSP1) (AIFM2) with an EC50 of 103 nM. iFSP1 selectively induces ferroptosis in GPX4-knockout cells which overexpressed FSP1. iFSP1 is able to sensitize a variety of human cancer cell lines to the ferroptosis inducer, such as (1S,3R)-RSL3 (HY-100218A)[1]. |
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| Related Catalog | |
| Target |
EC50: 103 nM (FSP1)[1] |
| In Vitro | iFSP1 (0.001-1 μM; 24 hours) inhibits the Gpx4-knockout cell growth as a dose-dependent manner, but does not inhibit the wild type cell growth. Treatment with the ferroptosis inhibitor Lip-1 protects GPX4- knockout cells from iFSP1-induced ferroptosis[1]. iFSP1 (0.001-1 μM; 24 hours) is less efficient than genetic deletion of FSP1, whereas iFSP1 treatment in the FSP1-knockout background had no additive effect to RSL3-induced ferroptosis[1]. iFSP1 (3 μM; 24 hours) treatment results in an obvious toxicity of RSL3 in a panel of genetically engineered (FSP1-knockout) human cancer cell lines[1]. AIFM2: the flavoprotein apoptosis-inducing factor mitochondria-associated 2 is a previously unrecognized anti-ferroptotic gene. AIFM2, which is renamed ferroptosis suppressor protein 1 (FSP1)[1] Cell Proliferation Assay[1] Cell Line: Wild-type and Gpx4-knockout Pfa1 or HT 1080 cells overexpressing FSP1–HA Concentration: 0.001-1 μM Incubation Time: 24 hours Result: Was toxic to cells that depend solely (no GPX4 expression detectable) on FSP1 function. Cell Viability Assay[1] Cell Line: NCl-H1437, NCl-H1437 FSP1 KO, U-373, U-373 FSP1 KO, MDA-MB-436, MDA-MB-436 FSP1 KO, SW620, SW620 FSP1 KO, MDA-MB-435S, MDA-MB-435S FSP1 KO, A549 and A549 FSP1 KO Concentration: 3 μM Incubation Time: 24 hours Result: Sensitized a variety of human cancer cell lines to the ferroptosis inducer (1S,3R)-RSL3. |
| References |
| Molecular Formula | C20H13N5 |
|---|---|
| Molecular Weight | 323.35100 |
| Exact Mass | 323.11700 |
| PSA | 90.90000 |
| LogP | 4.36966 |
| Hazard Codes | Xi |
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