- Shanghai Nianxing Industrial Co., Ltd
- China
- Product Name: Xylene Cyanole F
- Price:
- Purity: 70.0%
- Stocking Period: 10 Day
- Contact: Yang
- Shanghai Jizhi Biochemical Technology Co., Ltd
- China
- Product Name: Xylene Cyanole F
- Price: ￥198.0/5g
- Purity: 98.0%
- Stocking Period: Inquiry
- Contact: Liu jia
- Dayang Chem (Hangzhou) Co., Ltd.
- China
- Product Name: Xylene cyanole ff
- Price: $Inquiry/100g $Inquiry/1kg $Inquiry/100kg $Inquiry/1000kg
- Purity: 98.0%
- Stocking Period: Inquiry
- Contact: Ms Wang
- Henan Tianfu Chemical Co., Ltd.
- China
- Product Name: Xylene Cyanole FF
- Price: $Inquiry/1kg $Inquiry/25kg $Inquiry/1000kg $Inquiry/10ton
- Purity: 99.0%
- Stocking Period: Inquiry
- Contact: Anson
2650-17-1
2650-17-1 structure
- Name: Xylene Cyanole F
- Chemical Name: Xylene Cyanole FF
- CAS Number: 2650-17-1
- Molecular Formula: C25H27N2NaO6S2
- Molecular Weight: 538.611
- Catalog: Chemical reagent Organic reagent Cyanide/nitrile
- Create Date: 2018-02-10 08:00:00
- Modify Date: 2024-01-06 17:06:24
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Xylene Cyanol FF is an acid triphenylmethane dye. Xylene Cyanol FF can be used for histochemical staining of hemoglobin peroxidase or as a tracking dye for DNA sequencing in electrophoresis. Xylene Cyanol FF will be catalyzed by Fe and Al to accelerate oxidation under the addition of double oxidant hydrogen peroxide and potassium periodate. Xylene Cyanol FF thus enables the spectrophotometric determination of Fe and Al in the solution to be tested[1][2].
| Name | Xylene Cyanole FF |
|---|---|
| Synonyms |
Sodium 4-{(Z)-[4-(ethylamino)-3-methylphenyl][(4Z)-4-(ethyliminio)-3-methylcyclohexa-2,5-dien-1-ylidene]methyl}benzene-1,3-disulfonate
Xylene Cyanol FF Acid Blue 147 XYLENE CYANOL CYANOL BLUE EINECS 224-728-5 Sodium 4-{(Z)-[4-(ethylamino)-3-methylphenyl][(4Z)-4-(ethyliminio)-3-methyl-2,5-cyclohexadien-1-ylidene]methyl}-1,3-benzenedisulfonate 1,3-Benzenedisulfonic acid, 4-((4-(ethylamino)-3-methylphenyl)(4-(ethylimino)-3-methyl-2,5-cyclohexadien-1-ylidene)methyl)-, monosodium salt Xylene Cyanole F Sodium hydrogen 4-((4-(ethylamino)-m-tolyl)(4-(ethylimino)-3-methylcyclohexa-2,5-dien-1-ylidene)methyl)benzene-1,3-disulphonate CYANOL Blue 147 Acid blue 147,Cyanol FF,XC 1,3-Benzenedisulfonic acid, 4-[(Z)-[4-(ethylamino)-3-methylphenyl][(4Z)-4-(ethylimino)-3-methyl-2,5-cyclohexadien-1-ylidene]methyl]-, sodium salt (1:1) MFCD00040657 CYANOL FF |
| Description | Xylene Cyanol FF is an acid triphenylmethane dye. Xylene Cyanol FF can be used for histochemical staining of hemoglobin peroxidase or as a tracking dye for DNA sequencing in electrophoresis. Xylene Cyanol FF will be catalyzed by Fe and Al to accelerate oxidation under the addition of double oxidant hydrogen peroxide and potassium periodate. Xylene Cyanol FF thus enables the spectrophotometric determination of Fe and Al in the solution to be tested[1][2]. |
|---|---|
| Related Catalog | |
| In Vitro | Xylene Cyanol FF 能够在不同聚丙烯酰胺凝胶电泳示踪 DNA。操作如下[1]： 1.用于变性凝胶。 （1）配制凝胶：10% 的丙烯酰胺 (19:1，丙烯酰胺：双丙烯酰胺)，以及 8.3 M urea。使用条件为 55 ℃。 （2）配制电泳缓冲液：89 mM Tris，HCl，pH 8.0，89 mM 硼酸，2 mM EDTA (TBE)。 （3）配制上样缓冲液：10 mM NaOH，1 mM EDTA，以及 0.1% Xylene Cyanol FF。 （4）凝胶在 IBI 型号 STS 45 电泳装置上以 70 W (50 V/cm，恒定功率) 运行，或在 Hoefer SE 600 电泳装置上以 60 °C (31 V/cm，恒定电压) 运行。 （5）将凝胶置于 Whatman 3MM 纸上干燥并在 X 射线胶片上曝光。曝光时间长达 15 小时。2.用于变性凝胶。 （1）配制凝胶：含有 8% 的丙烯酰胺 (19:1，丙烯酰胺：双丙烯酰胺)。 （2）配制 DNA 悬液：含有 40 mM TrisβHCl，pH 8.0、20 mM 乙酸、2 mM EDTA 和 12.5 mM 乙酸镁 (TAEMg)。 （3）煮沸 DNA 悬液并缓慢冷却至 16 °C。 （4）配制染色液：含有 TAEMg、50% 甘油、0.02% 溴酚蓝和 0.02% Xylene Cyanol FF。用该染色液将样品调至 10 µL 的最终体积。 （5）凝胶在 Hoefer SE-600 凝胶电泳装置上以 11 V/cm 的电压在 16 °C 下运行，并暴露于 X 射线胶片长达 15 小时或用 Stainsall (HY-D0987) 染料染色。 |
| References |
| Melting Point | 295°C |
|---|---|
| Molecular Formula | C25H27N2NaO6S2 |
| Molecular Weight | 538.611 |
| Exact Mass | 538.120850 |
| PSA | 152.72000 |
| LogP | 6.59110 |
| Storage condition | Store at RT. |
| Symbol |
GHS07 |
|---|---|
| Signal Word | Warning |
| Hazard Statements | H315-H319-H335 |
| Precautionary Statements | P261-P305 + P351 + P338 |
| Personal Protective Equipment | dust mask type N95 (US);Eyeshields;Gloves |
| Hazard Codes | Xi |
| Risk Phrases | R36/37/38 |
| Safety Phrases | S26-S36-S24/25 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| HS Code | 32041900 |
| HS Code | 32041900 |
|---|