Shanghai Nianxing Industrial Co., Ltd
China
Product Name: H2DCFDA
Price:
Purity: 98.0%
Stocking Period: 10 Day
Contact: Yang

Shanghai Jizhi Biochemical Technology Co., Ltd
China
Product Name: H2DCFDA
Price: ￥3448.0/100mg ￥988.0/25mg
Purity: 95.0%
Stocking Period: Inquiry
Contact: Liu jia

ShangHai DC Chemicals Co.,Ltd
China
Product Name: H2DCFDA
Price: ￥Inquiry/1g
Purity: 98.9%
Stocking Period: 1 Day
Contact: Tony Lai

DC Chemicals Limited
China
Product Name: H2DCFDA
Price: $Inquiry/100mg $Inquiry/250mg $Inquiry/500mg
Purity: 98.0%
Stocking Period: 3 Day
Contact: Tony Cao

Dayang Chem (Hangzhou) Co., Ltd.
China
Product Name: 2-(3,6-DIACETOXY-2,7-DICHLORO-9H-XANTHEN-9-YL)BENZOIC ACID
Price: $Inquiry/100g $Inquiry/1kg $Inquiry/100kg $Inquiry/1000kg
Purity: 98.0%
Stocking Period: Inquiry
Contact: Ms Wang

4091-99-0

4091-99-0 structure

4091-99-0 structure

Name: H2DCFDA
Chemical Name: 2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid
CAS Number: 4091-99-0
Molecular Formula: C₂₄H₁₆Cl₂O₇
Molecular Weight: 487.286
Catalog: Dye Reagents
Create Date: 2018-05-07 08:00:00
Modify Date: 2024-01-02 07:25:50
H2DCFDA is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS).

Name	2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid
Synonyms	2,7-Dichlorodihydrofluorescein diacetate H2DCFDA [2',7'-Dichlorodihydrofluorescein diacetate] MFCD00128955 2-(3,6-Diacetoxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid Leucodiacetyldichlorofluorescein H2DCFDA 2',7'-dichlorofluorescin diacetate Benzoic acid, 2-[3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]- DCFH

Description	H2DCFDA is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS).
Related Catalog	Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others
In Vitro	Analysis of H2DCFDA probe oxidation by means of flow cytometry technique showed that the basal level of ROS in difESCs is about 6–7 times larger than that in embryonic stem cells (ESCs). Both H2DCFDA and DCFDA probes are non-fluorescent in their initial form but they undergo multistep conversion inside the cell that results in the formation of fluorescent product dichlorofluorescein (DCF). The only difference between these two probes is that the conversion of H2DCFDA involves oxidation. Therefore, fluorescence of the H2DCFDA-treated cells depends on the intracellular ROS level, in contrast to fluorescence associated with the DCFDA probe. Surprisingly, flow cytometry analysis shows that the difference between fluorescence levels of ESCs and difESCs loaded with H2DCFDA is close to the difference between the signals from DCFDA-treated cells. The latter indicates a ROS-independent reason for low fluorescent signal of oxidized H2DCFDA in ESCs[1].
Kinase Assay	H2DCFDA is dissolved in DMSO to obtain a 10 mM stock solutions and further diluted before use[1]. ROS Measurements[1] For the detection of intracellular ROS level ,ROS-sensitive probe H2DCFDA is used. Adherent cells (ESCs, difESCs, eMSCs, HeLa, U118) are incubated with 5 µM staining solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, and immediately analyzed with flow cytometer. Lymphocytes, both control and PHA-activated, are resuspended in PBS, incubated with 5 µM of H2DCFDA in the dark for 30 min at 37°C, and immediately analyzed. Along with the H2DCFDA probe, if indicated, ROS-insensitive modification of the fluorescein dye DCFDA is used. The staining procedure is the same as for the H2DCFDA[1].
References	[1]. Lyublinskaya OG, et al. Redox environment in stem and differentiated cells: A quantitative approach. Redox Biol. 2017 Aug;12:758-769.

Density	1.5±0.1 g/cm3
Boiling Point	593.5±50.0 °C at 760 mmHg
Melting Point	209-210ºC(lit.)
Molecular Formula	C₂₄H₁₆Cl₂O₇
Molecular Weight	487.286
Flash Point	312.7±30.1 °C
Exact Mass	486.027313
PSA	99.13000
LogP	4.62
Vapour Pressure	0.0±1.8 mmHg at 25°C
Index of Refraction	1.635
Storage condition	2-8°C

Symbol	GHS07
Signal Word	Warning
Hazard Statements	H315-H319-H335
Precautionary Statements	P261-P305 + P351 + P338
Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes	Xi
Risk Phrases	36/37/38
Safety Phrases	S22-S24/25
RIDADR	NONH for all modes of transport
WGK Germany	3.0
HS Code	2932999099

Precursor 0
DownStream 2
106070-31-9 76-54-0

HS Code	2932999099
Summary	2932999099. other heterocyclic compounds with oxygen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%