76-54-0

76-54-0 structure

Name: dichlorofluorescein
Chemical Name: 2',7'-dichlorofluorescein
CAS Number: 76-54-0
Molecular Formula: C₂₀H₁₀Cl₂O₅
Molecular Weight: 401.196
Catalog: analytical chemistry Analytical reagent Common analytical reagents
Create Date: 2018-06-05 08:00:00
Modify Date: 2025-08-20 14:58:26
2',7'-Dichlorofluorescein acts as a fluorescent probe (Ex=496 nm and Em=525 nm) for reactive oxygen species (ROS) measurement[1].

Name	2',7'-dichlorofluorescein
Synonyms	Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 2',7'-dichloro-3',6'-dihydroxy- EINECS 200-968-6 dichlorofluorescein 2',7'-dichloro-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one 2',7'-Dichloro-3',6'-dihydroxy-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 2',7'-dichloro-3',6'-dihydroxy- 2‘,7‘-Dichlorofluorescein MFCD00005047

Description	2',7'-Dichlorofluorescein acts as a fluorescent probe (Ex=496 nm and Em=525 nm) for reactive oxygen species (ROS) measurement[1].
Related Catalog	Research Areas >> Cancer
References	[1]. Reiniers MJ, et al. Preparation and Practical Applications of 2',7'-Dichlorodihydrofluorescein in Redox Assays. Anal Chem. 2017 Apr 4;89(7):3853-3857. [2]. Xiuping Chen, et al. 2',7'-Dichlorodihydrofluorescein as a fluorescent probe for reactive oxygen species measurement: Forty years of application and controversy. Free Radic Res. 2010 Jun;44(6):587-604.

Density	1.8±0.1 g/cm3
Boiling Point	634.4±55.0 °C at 760 mmHg
Melting Point	280 °C (dec.)(lit.)
Molecular Formula	C₂₀H₁₀Cl₂O₅
Molecular Weight	401.196
Flash Point	337.4±31.5 °C
Exact Mass	399.990540
PSA	75.99000
LogP	4.10
Vapour Pressure	0.0±1.9 mmHg at 25°C
Index of Refraction	1.797
Stability	Stable. Incompatible with strong oxidizing agents.

Symbol	GHS02, GHS07
Signal Word	Danger
Hazard Statements	H225-H319-H336
Precautionary Statements	P210-P261-P305 + P351 + P338
Personal Protective Equipment	dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes	Xi: Irritant;
Risk Phrases	R36/37/38
Safety Phrases	S26-S36
RIDADR	UN 1219 3/PG 2
WGK Germany	3
RTECS	NT8050000
Hazard Class	3.0
HS Code	3204200000

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Literature: EP1493781 A1, ; Page 113 ;

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Literature: Chemical Communications, , vol. 47, # 5 p. 1437 - 1439

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Literature: Journal of the American Chemical Society, , vol. 51, p. 3274

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Literature: Journal of the American Chemical Society, , vol. 51, p. 3274

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Literature: Photochemistry and Photobiology, , vol. 84, # 5 p. 1238 - 1243

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Literature: European Journal of Inorganic Chemistry, , # 12 p. 1974 - 1980

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Literature: DE230737 ; Fortschr. Teerfarbenfabr. Verw. Industriezweige, vol. 10, p. 243

HS Code	2932999099
Summary	2932999099. other heterocyclic compounds with oxygen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%