53213-82-4
53213-82-4 structure
- Name: 3,3'-Dihexyloxacarbocyanine iodide
- Chemical Name: 3,3'-dihexyloxacarbocyanine iodide
- CAS Number: 53213-82-4
- Molecular Formula: C29H37IN2O2
- Molecular Weight: 572.52100
- Catalog: Chemical reagent Organic reagent Cyanide/nitrile
- Create Date: 2018-08-25 16:30:19
- Modify Date: 2025-08-21 11:46:31
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3,3'-Dihexyloxacarbocyanine iodide is a carbocyanine dye which can be used to monitor changes in mitochondrial membrane potential.
| Name | 3,3'-dihexyloxacarbocyanine iodide |
|---|---|
| Synonyms |
MFCD00011954
DiOC6(3) [3,3'-Dihexyloxacarbocyanine iodide] 3-hexyl-2-[3-(3-hexyl-1,3-benzoxazol-3-ium-2-yl)prop-2-enylidene]-1,3-benzoxazole,iodide 3-hexyl-2-[3-(3-hexyl-2(3H)benzoxazolylidene)-1-propenyl]benzoxazolium iodide |
| Description | 3,3'-Dihexyloxacarbocyanine iodide is a carbocyanine dye which can be used to monitor changes in mitochondrial membrane potential. |
|---|---|
| Related Catalog | |
| In Vitro | Addition of cells to the cuvette containing 0.25 μM 3,3'-Dihexyloxacarbocyanine iodide (DiOCg(3)) leads to an increase in fluorescence, equilibration is rapid and is complete by 4 min. When f-met-leu-phe (10-7 M) is added to neutrophils preequilibrated with 3,3'-Dihexyloxacarbocyanine iodide, there is a short lag period of 5 seconds. The lag period is followed by a rapid loss of fluorescence. Examination of 3,3'-Dihexyloxacarbocyanine iodide loaded neutrophils using fluorescence microscopy demonstrates that in resting neutrophils the brightest fluorescence is associated with long thin organelles which corresponds to the distribution of mitochondria[1]. |
| Cell Assay | Neutrophils (2.5×106/mL) are preequilibrated in 0.25 μM 3,3'-Dihexyloxacarbocyanine iodide (DiOCg(3)) for 5 min at 37°C in a 1 cm path length cuvette. Final volume is 2 mL. Fluorescence measurements are made in a spectrofluorimeter (emission wavelength: 510 nm, excitation wavelength: 470 nm). Stimuli are injected by a syringe through an injection port on the spectrofluorimeter, and their volumes never exceed 5% (v/v)[1]. |
| References |
| Melting Point | 219-221 °C(lit.) |
|---|---|
| Molecular Formula | C29H37IN2O2 |
| Molecular Weight | 572.52100 |
| Exact Mass | 572.19000 |
| PSA | 35.09000 |
| LogP | 4.20760 |
| Symbol |
GHS07 |
|---|---|
| Signal Word | Warning |
| Hazard Statements | H315-H319-H335 |
| Precautionary Statements | P261-P305 + P351 + P338 |
| Personal Protective Equipment | dust mask type N95 (US);Eyeshields;Gloves |
| Hazard Codes | Xi |
| Risk Phrases | R36/37/38 |
| Safety Phrases | S26-S36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| RTECS | DM5285250 |