Dayang Chem (Hangzhou) Co., Ltd.
China
Product Name: 3,3'-dihexyloxacarbocyanine iodide
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53213-82-4

53213-82-4 structure

53213-82-4 structure

Name: 3,3'-Dihexyloxacarbocyanine iodide
Chemical Name: 3,3'-dihexyloxacarbocyanine iodide
CAS Number: 53213-82-4
Molecular Formula: C₂₉H₃₇IN₂O₂
Molecular Weight: 572.52100
Catalog: Chemical reagent Organic reagent Cyanide/nitrile
Create Date: 2018-08-25 16:30:19
Modify Date: 2024-01-02 11:20:38
3,3'-Dihexyloxacarbocyanine iodide is a carbocyanine dye which can be used to monitor changes in mitochondrial membrane potential.

Name	3,3'-dihexyloxacarbocyanine iodide
Synonyms	MFCD00011954 DiOC6(3) [3,3'-Dihexyloxacarbocyanine iodide] 3-hexyl-2-[3-(3-hexyl-1,3-benzoxazol-3-ium-2-yl)prop-2-enylidene]-1,3-benzoxazole,iodide 3-hexyl-2-[3-(3-hexyl-2(3H)benzoxazolylidene)-1-propenyl]benzoxazolium iodide

Description	3,3'-Dihexyloxacarbocyanine iodide is a carbocyanine dye which can be used to monitor changes in mitochondrial membrane potential.
Related Catalog	Signaling Pathways >> Others >> Others Research Areas >> Others Dye Reagents
In Vitro	Addition of cells to the cuvette containing 0.25 μM 3,3'-Dihexyloxacarbocyanine iodide (DiOCg(3)) leads to an increase in fluorescence, equilibration is rapid and is complete by 4 min. When f-met-leu-phe (10-7 M) is added to neutrophils preequilibrated with 3,3'-Dihexyloxacarbocyanine iodide, there is a short lag period of 5 seconds. The lag period is followed by a rapid loss of fluorescence. Examination of 3,3'-Dihexyloxacarbocyanine iodide loaded neutrophils using fluorescence microscopy demonstrates that in resting neutrophils the brightest fluorescence is associated with long thin organelles which corresponds to the distribution of mitochondria[1].
Cell Assay	Neutrophils (2.5×106/mL) are preequilibrated in 0.25 μM 3,3'-Dihexyloxacarbocyanine iodide (DiOCg(3)) for 5 min at 37°C in a 1 cm path length cuvette. Final volume is 2 mL. Fluorescence measurements are made in a spectrofluorimeter (emission wavelength: 510 nm, excitation wavelength: 470 nm). Stimuli are injected by a syringe through an injection port on the spectrofluorimeter, and their volumes never exceed 5% (v/v)[1].
References	[1]. Korchak HM, et al. A carbocyanine dye, DiOC6(3), acts as a mitochondrial probe in human neutrophils. Biochem Biophys Res Commun. 1982 Oct 29;108(4):1495-501.

Melting Point	219-221 °C(lit.)
Molecular Formula	C₂₉H₃₇IN₂O₂
Molecular Weight	572.52100
Exact Mass	572.19000
PSA	35.09000
LogP	4.20760

Symbol	GHS07
Signal Word	Warning
Hazard Statements	H315-H319-H335
Precautionary Statements	P261-P305 + P351 + P338
Personal Protective Equipment	dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes	Xi
Risk Phrases	R36/37/38
Safety Phrases	S26-S36
RIDADR	NONH for all modes of transport
WGK Germany	3
RTECS	DM5285250