407587-33-1

407587-33-1 structure

Name: CaCCinh-A01
Chemical Name: 6-t-butyl-2-(furan-2-carboxamido)-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carboxylic acid
CAS Number: 407587-33-1
Molecular Formula: C₁₈H₂₁NO₄S
Molecular Weight: 347.42900
Catalog: Signaling Pathways Membrane Transporter/Ion Channel Chloride Channel
Create Date: 2016-10-02 03:45:02
Modify Date: 2024-01-05 17:54:29
CaCCinh-A01 is an inhibitor of both TMEM16A and calcium-activated chloride channel (CaCC) with IC50s of 2.1 and 10 μM, respectively.

Name	6-t-butyl-2-(furan-2-carboxamido)-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carboxylic acid
Synonyms	CaCCinh-A01

Description	CaCCinh-A01 is an inhibitor of both TMEM16A and calcium-activated chloride channel (CaCC) with IC50s of 2.1 and 10 μM, respectively.
Related Catalog	Signaling Pathways >> Membrane Transporter/Ion Channel >> Chloride Channel Research Areas >> Cancer Research Areas >> Inflammation/Immunology
Target	IC50: 2.1 μM (TMEM16A)[1], 10 μM (CaCC)[2]
In Vitro	30 μM CaCCinh-A01 and 100 μM tannic acid strongly inhibit CaCC current following ATP stimulation[1]. Calcium-dependent chloride current is reduced by 38±14, 66±10, and 91±1% by 0.1, 1, and 10 μM CaCCinh-A01, respectively. ATP-induced short-circuit currents are reduced by 38±7 and 78±3% at 10 and 30 μM CaCCinh-A01, respectively[2].
Cell Assay	Each well of a 96-well plate is washed three times with PBS (200 μL/wash), leaving 50 μL of PBS. Test compounds (including CaCCinh-A01) (0.5 μL) are added to each well at 25 μM final concentration. After 10 min, 96-well plates are transferred to a plate reader for fluorescence assay. Each well is assayed individually for TMEM16A-mediated I- influx by recording fluorescence continuously (400 ms/point) for 2 s (base line), then 50 μL of a 140 mM I- solution containing 200 μM ATP is added. The initial rate of I- influx is computed from fluorescence data by nonlinear regression[1].
References	[1]. TMEM16A inhibitors reveal TMEM16A as a minor component of calcium-activated chloridechannel conductance in airway and intestinal epithelial cells. J Biol Chem. 2011 Jan 21;286(3):2365-74. [2]. De La Fuente R, et al. Small-molecule screen identifies inhibitors of a human intestinal calcium-activated chloridechannel. Mol Pharmacol. 2008 Mar;73(3):758-68.

Molecular Formula	C₁₈H₂₁NO₄S
Molecular Weight	347.42900
Exact Mass	347.11900
PSA	107.78000
LogP	4.51560
Storage condition	-20℃

RIDADR	NONH for all modes of transport

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