| Name | 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein |
|---|---|
| Synonyms |
CFSE[5-(and 6)-Cboxyfluorescein diacetate
5-or6-(N-Succinimidyloxycbonyl)-fluorescein3',6'-diacetate Carboxyfluorescein diacetate succinimidyl ester 5-Cboxyfluorescein 3',6'-Diacetate N-SucciniMidyl Ester 5-CBOXYFLUORESCEIN DIACETATE-N-HYDROXYSUCCINIMIDE ESTER 5-CFDA N-succinimidyl ester (CFSE) MFCD00037466 5-CBOXYFLUORESCEIN-NHS DIACETATE (HPCE) 2,5-Dioxopyrrolidin-1-ylacetatecompoundwith3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyldiacetate(1:1) 5-CFDA N-succinmidyl este Cboxyfluorescein succinimidyl ester 5-CBOXY-DI-O-ACETYLFLUORESCEIN N-SUCCINIMIDYL ESTER 3'',6''-O,O''-Diacetyl-5-(N-succinimidyloxycbonyl)-fluorescein NHS ester 5-CFDA N-succinimidyl ester 5-FAM-NHS DIACETATE 5-CBOXY-FLUORESCEIN DIACETATE N-SUCCINIMIDYL ESTER 5-CFDA, SE [5-Cboxyfluorescein diacetate, SE] Fluorescent pH Indicator Cellstain-Cfse Cell proliferation tracer fluorescence probe 5-CFDA (SE [5-Cboxyfluorescein diacetate 5-CFDA, SE [5-Cboxyfluorescein diacetate, succiniMidyl ester] 5-CFDA N-succinmidyl ester 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester CFSE (CFDA-SE) CFSE (Synonyms: 5-Cboxyfluorescein diacetate succinimidyl ester 5-or6-(N-Succiimidyloxycbonyl)-3',6'-O,O'-diacethylfluorescein 5-CFDA N-succinmidyl ester) MCE CFDA SE 5-or 6-(N-Succinimidyloxycbonyl)-3', 6'-O,O'-diacetylfluorescein (CFSE) Spiro[isobenzofuran-1(3h),9′-[9h]xanthene], 2,5-pyrrolidinedione derivative Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-cboxylic acid, 3',6'-bis(acetyloxy)-3-oxo-, 2,5-dioxo-1-pyrrolidinyl ester Cell Proliferation Tracer Fluorescent Probe (CFDASE) 5-FAM DA SE Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-cboxylicacid, 3',6'-bis(acetyloxy)-3-oxo-, 2,5-dioxo-1-pyrrolidinyl ester 5-(N-SUCCINIMIDYLOXYCBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN 5-CBOXYFLUORESCEIN DIACETATE N-SUCCINIMIDYL ESTER Cboxyfluorescein diacetate, succinimidyl ester 1-[[[3',6'-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen] CFSE [5-(and 6)-Cboxyfluorescein diacetate, succinimidyl ester] 5-Cboxyfluorescein diacetate, succinimidyl ester(CFDA) 5-cboxyfluorescein diacetate N-succinimidyl este 5-CBOXYFLUORESCEIN DIACETATE-N-HYDROXY-SUCCINIMIDE ESTER ReadiUse CFSE [5-(and 6)-Cboxyfluorescein diacetate, succinimidyl ester] 5-{[(2,5-Dioxo-1-pyrrolidinyl)oxy]carbonyl}-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-diyl diacetate CFSE 5-CFDA N-succinimidyl ester (CFSE), Fluorogenic esterase substrate CBOXYFLUORESCEIN DIACETATE, NHS ESTER CFSE, 10 mM in DMSO 3',6'-Bis(acetyloxy)-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-cboxylic Acid 2,5-Dioxo-1-pyrrolidinyl Ester 5(6)-CFDA (SE [5(6)-Carboxyfluorescein diacetate 5-cboxy' Uorescein Diacetate Succinimidyl Ester 5-Carboxyfluorescein diacetate N-Hydroxysuccinimide Ester |
| Description | CFSE is a fluorescent dye which can track the cell division. |
|---|---|
| Related Catalog | |
| In Vitro | CFSE is a fluorescent dye which can track the cell division[1]. To examine the ability of immune cells to migrate into live slices, a single-cell suspension of autologous splenocytes or PBMC is isolated, labeling them with CFSE, and adding them to the top of the slices on day 2 of culture. After 6 additional days of culture, the CFSE-labeling immune cells (green) are found to have migrated throughout the slices. No positive cells are found in the slices without the addition of CFSE-labeling immune cells[2]. |
| Cell Assay | Single-cell suspensions of splenocytes (or PBMC) are stained with 1 µM CFSE in PBS for 9 min at 37°C, combined with 20 mL of 10% FBS RPMI-1640 medium at RT for 2 min, centrifuged, washed, and counted. 2×106 CFSE-labeling splenocytes are added to the top of the slices in 24-well membrane culture insert[2]. |
| References |
| Density | 1.6±0.1 g/cm3 |
|---|---|
| Boiling Point | 757.9±70.0 °C at 760 mmHg |
| Melting Point | 152-154ºC(lit.) |
| Molecular Formula | C29H19NO11 |
| Molecular Weight | 557.461 |
| Flash Point | 412.2±35.7 °C |
| Exact Mass | 557.095825 |
| PSA | 151.81000 |
| LogP | 0.50 |
| Vapour Pressure | 0.0±2.6 mmHg at 25°C |
| Index of Refraction | 1.701 |
| Storage condition | -20°C |
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| Hazard Codes | Xi |
| RIDADR | NONH for all modes of transport |