5-Carboxyfluorescein diacetate N-succinimidyl ester
5-Carboxyfluorescein diacetate N-succinimidyl ester structure
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Common Name | 5-Carboxyfluorescein diacetate N-succinimidyl ester | ||
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| CAS Number | 150347-59-4 | Molecular Weight | 557.461 | |
| Density | 1.6±0.1 g/cm3 | Boiling Point | 757.9±70.0 °C at 760 mmHg | |
| Molecular Formula | C29H19NO11 | Melting Point | 152-154ºC(lit.) | |
| MSDS | USA | Flash Point | 412.2±35.7 °C | |
Use of 5-Carboxyfluorescein diacetate N-succinimidyl esterCFSE is a fluorescent dye which can track the cell division. |
This table lists Chinese names, IUPAC names and various aliases of this chemical substance.
| Name | 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein |
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| Synonym | More Synonyms |
This table contains bioactivity, target information and biological‑assay experimental data of this compound.
| Description | CFSE is a fluorescent dye which can track the cell division. |
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| Related Catalog | |
| In Vitro | CFSE is a fluorescent dye which can track the cell division[1]. To examine the ability of immune cells to migrate into live slices, a single-cell suspension of autologous splenocytes or PBMC is isolated, labeling them with CFSE, and adding them to the top of the slices on day 2 of culture. After 6 additional days of culture, the CFSE-labeling immune cells (green) are found to have migrated throughout the slices. No positive cells are found in the slices without the addition of CFSE-labeling immune cells[2]. |
| Cell Assay | Single-cell suspensions of splenocytes (or PBMC) are stained with 1 µM CFSE in PBS for 9 min at 37°C, combined with 20 mL of 10% FBS RPMI-1640 medium at RT for 2 min, centrifuged, washed, and counted. 2×106 CFSE-labeling splenocytes are added to the top of the slices in 24-well membrane culture insert[2]. |
| References |
This table shows physicochemical parameters including melting point, boiling point, density, solubility and optical rotation. Missing data is marked as N/A.
| Density | 1.6±0.1 g/cm3 |
|---|---|
| Boiling Point | 757.9±70.0 °C at 760 mmHg |
| Melting Point | 152-154ºC(lit.) |
| Molecular Formula | C29H19NO11 |
| Molecular Weight | 557.461 |
| Flash Point | 412.2±35.7 °C |
| Exact Mass | 557.095825 |
| PSA | 151.81000 |
| LogP | 0.50 |
| Vapour Pressure | 0.0±2.6 mmHg at 25°C |
| Index of Refraction | 1.701 |
| Storage condition | -20°C |
This table covers safety warnings, protective measures, incompatible substances and disposal guidelines for this compound.
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| Hazard Codes | Xi |
| RIDADR | NONH for all modes of transport |
This table lists relevant public references with title, journal and publication metadata for this compound.
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MHC class II presentation is controlled by the lysosomal small GTPase, Arl8b.
J. Immunol. 194(5) , 2079-88, (2015) Dendritic cells (DCs) are specialized APCs with the ability to prime naive T cells. DCs first sample Ags from the environment and then orchestrate their processing and loading onto MHC class II (MHC I... |
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Coculture of peripheral blood-derived mesenchymal stem cells and endothelial progenitor cells on strontium-doped calcium polyphosphate scaffolds to generate vascularized engineered bone.
Tissue Eng. Part A 21(5-6) , 948-59, (2015) Vascularization of engineered bone tissue is critical for ensuring its survival after implantation and it is the primary factor limiting its clinical use. A promising approach is to prevascularize bon... |
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Olanzapine inhibits proliferation, migration and anchorage-independent growth in human glioblastoma cell lines and enhances temozolomide's antiproliferative effect.
J. Neurooncol. 122(1) , 21-33, (2015) The poor prognosis of patients with glioblastoma fuels the search for more effective therapeutic compounds. We previously hypothesised that the neuroleptic olanzapine may enhance antineoplastic effect... |
This table collects all English aliases, systematic names and CAS‑related synonyms of the compound.
| 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester |
| Carboxyfluorescein diacetate succinimidyl ester |
| 5(6)-CFDA (SE [5(6)-Carboxyfluorescein diacetate |
| MFCD00037466 |
| 5-Carboxyfluorescein diacetate N-Hydroxysuccinimide Ester |
| 5-{[(2,5-Dioxo-1-pyrrolidinyl)oxy]carbonyl}-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-diyl diacetate |
| 5-CFDA N-succinimidyl ester |
| 5-Carboxyfluorescein diacetate N-succinimidyl ester |
| CFSE |
This section contains frequently‑asked‑questions about this compound, including basic parameters, properties, storage conditions and corresponding answers.
| Q: What is the CAS number of 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein? |
| A: CAS number of 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein is 150347-59-4. |
| Q: What is the polar surface area (PSA) of 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein? |
| A: Polar surface area (PSA) of 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein is 151.81000. |
| Q: What is the molecular formula of 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein? |
| A: Molecular formula of 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein is C29H19NO11. |
| Q: What are the uses of 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein? |
| A: Main uses of 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein: CFSE is a fluorescent dye which can track the cell division. |
| Q: What are the storage conditions for 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein? |
| A: Storage conditions for 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein: -20°C. |
| Q: What is the LogP of 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein? |
| A: LogP of 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein is 0.50. |
| Q: What is the boiling point of 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein? |
| A: Boiling point of 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein is 757.9±70.0 °C at 760 mmHg. |
| Q: What is the molecular weight of 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein? |
| A: Molecular weight of 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein is 557.461. |
| Q: What is the English name of 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein? |
| A: The English name of 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein is 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein. |
| Q: What is the melting point of 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein? |
| A: Melting point of 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein is 152-154ºC(lit.). |
This table lists manufacturers and suppliers of this compound, including vendor names and product supply reference information.
| Shanghai Nianxing Industrial Co., Ltd |
| Dayang Chem (Hangzhou) Co., Ltd. |