68542-93-8

68542-93-8 structure

Name: Z-Gly-Pro-AMC
Chemical Name: benzyl N-[2-[(2S)-2-[(4-methyl-2-oxochromen-7-yl)carbamoyl]pyrrolidin-1-yl]-2-oxoethyl]carbamate
CAS Number: 68542-93-8
Molecular Formula: C₂₅H₂₅N₃O₆
Molecular Weight: 463.48300
Catalog: Biochemical Amino acids and their derivatives Proline derivatives
Create Date: 2019-01-01 07:57:00
Modify Date: 2024-01-02 20:34:00
Z-Gly-Pro-AMC is a fluorogenic substrate. Z-Gly-Pro-AMC is hydrolyzed by prolyl endopeptidase to generate highly fluorescent 7-amido-4-methylcoumarin (HY-D0027). (λex=380 nm, λem=465 nm)[1].

Name	benzyl N-[2-[(2S)-2-[(4-methyl-2-oxochromen-7-yl)carbamoyl]pyrrolidin-1-yl]-2-oxoethyl]carbamate
Synonyms	Z-GP-AMC Z-Gly-Pro-AMC Z-Gly-propmecounh N-Carbobenzoxyglycylprolyl-4-methylcoumarinyl amide Z-Gly-pro-NH-Mec MFCD00038082

Description	Z-Gly-Pro-AMC is a fluorogenic substrate. Z-Gly-Pro-AMC is hydrolyzed by prolyl endopeptidase to generate highly fluorescent 7-amido-4-methylcoumarin (HY-D0027). (λex=380 nm, λem=465 nm)[1].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Z-Gly-Pro-AMC assay[1]: 1. Make a 5 μL of plasma sample is pre-incubated with 10 μL of 250 nM FAP inhibitor, 10 μL of 250 nM PREP inhibitor or 10 μL of 0.0025% (v/v) DMSO for 15 min at 37 ℃. 2. Add 35 μL pre-heated Z-Gly-Pro-AMC (380 μM diluted in buffer) to obtain a final concentration of 266 μM. 3. Fluorescence is measured kinetically for 30 min at 37 ℃.
References	[1]. Bracke A, et, al. The development and validation of a combined kinetic fluorometric activity assay for fibroblast activation protein alpha and prolyl oligopeptidase in plasma. Clin Chim Acta. 2019 Aug;495:154-160.

Safety Phrases	S22;S24/25
WGK Germany	3