68542-93-8
68542-93-8 structure
- Name: Z-Gly-Pro-AMC
- Chemical Name: benzyl N-[2-[(2S)-2-[(4-methyl-2-oxochromen-7-yl)carbamoyl]pyrrolidin-1-yl]-2-oxoethyl]carbamate
- CAS Number: 68542-93-8
- Molecular Formula: C25H25N3O6
- Molecular Weight: 463.48300
- Catalog: Biochemical Amino acids and their derivatives Proline derivatives
- Create Date: 2019-01-01 07:57:00
- Modify Date: 2025-08-25 14:52:48
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Z-Gly-Pro-AMC is a fluorogenic substrate. Z-Gly-Pro-AMC is hydrolyzed by prolyl endopeptidase to generate highly fluorescent 7-amido-4-methylcoumarin (HY-D0027). (λex=380 nm, λem=465 nm)[1].
| Name | benzyl N-[2-[(2S)-2-[(4-methyl-2-oxochromen-7-yl)carbamoyl]pyrrolidin-1-yl]-2-oxoethyl]carbamate |
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| Synonyms |
Z-GP-AMC
Z-Gly-Pro-AMC Z-Gly-propmecounh N-Carbobenzoxyglycylprolyl-4-methylcoumarinyl amide Z-Gly-pro-NH-Mec MFCD00038082 |
| Description | Z-Gly-Pro-AMC is a fluorogenic substrate. Z-Gly-Pro-AMC is hydrolyzed by prolyl endopeptidase to generate highly fluorescent 7-amido-4-methylcoumarin (HY-D0027). (λex=380 nm, λem=465 nm)[1]. |
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| Related Catalog | |
| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Z-Gly-Pro-AMC assay[1]: 1. Make a 5 μL of plasma sample is pre-incubated with 10 μL of 250 nM FAP inhibitor, 10 μL of 250 nM PREP inhibitor or 10 μL of 0.0025% (v/v) DMSO for 15 min at 37 ℃. 2. Add 35 μL pre-heated Z-Gly-Pro-AMC (380 μM diluted in buffer) to obtain a final concentration of 266 μM. 3. Fluorescence is measured kinetically for 30 min at 37 ℃. |
| References |
| Density | 1.358g/cm3 |
|---|---|
| Boiling Point | 770.8ºC at 760 mmHg |
| Melting Point | 96-102ºC(lit.) |
| Molecular Formula | C25H25N3O6 |
| Molecular Weight | 463.48300 |
| Flash Point | 420ºC |
| Exact Mass | 463.17400 |
| PSA | 117.95000 |
| LogP | 3.35910 |
| Index of Refraction | 1.635 |
| Storage condition | −20°C |
| Safety Phrases | S22;S24/25 |
|---|---|
| WGK Germany | 3 |