Z-Gly-Pro-AMC structure
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Common Name | Z-Gly-Pro-AMC | ||
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CAS Number | 68542-93-8 | Molecular Weight | 463.48300 | |
Density | 1.358g/cm3 | Boiling Point | 770.8ºC at 760 mmHg | |
Molecular Formula | C25H25N3O6 | Melting Point | 96-102ºC(lit.) | |
MSDS | N/A | Flash Point | 420ºC |
Use of Z-Gly-Pro-AMCZ-Gly-Pro-AMC is a fluorogenic substrate. Z-Gly-Pro-AMC is hydrolyzed by prolyl endopeptidase to generate highly fluorescent 7-amido-4-methylcoumarin (HY-D0027). (λex=380 nm, λem=465 nm)[1]. |
Name | benzyl N-[2-[(2S)-2-[(4-methyl-2-oxochromen-7-yl)carbamoyl]pyrrolidin-1-yl]-2-oxoethyl]carbamate |
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Synonym | More Synonyms |
Description | Z-Gly-Pro-AMC is a fluorogenic substrate. Z-Gly-Pro-AMC is hydrolyzed by prolyl endopeptidase to generate highly fluorescent 7-amido-4-methylcoumarin (HY-D0027). (λex=380 nm, λem=465 nm)[1]. |
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Related Catalog | |
In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Z-Gly-Pro-AMC assay[1]: 1. Make a 5 μL of plasma sample is pre-incubated with 10 μL of 250 nM FAP inhibitor, 10 μL of 250 nM PREP inhibitor or 10 μL of 0.0025% (v/v) DMSO for 15 min at 37 ℃. 2. Add 35 μL pre-heated Z-Gly-Pro-AMC (380 μM diluted in buffer) to obtain a final concentration of 266 μM. 3. Fluorescence is measured kinetically for 30 min at 37 ℃. |
References |
Density | 1.358g/cm3 |
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Boiling Point | 770.8ºC at 760 mmHg |
Melting Point | 96-102ºC(lit.) |
Molecular Formula | C25H25N3O6 |
Molecular Weight | 463.48300 |
Flash Point | 420ºC |
Exact Mass | 463.17400 |
PSA | 117.95000 |
LogP | 3.35910 |
Index of Refraction | 1.635 |
Storage condition | −20°C |
Safety Phrases | S22;S24/25 |
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WGK Germany | 3 |
Z-GP-AMC |
Z-Gly-Pro-AMC |
Z-Gly-propmecounh |
N-Carbobenzoxyglycylprolyl-4-methylcoumarinyl amide |
Z-Gly-pro-NH-Mec |
MFCD00038082 |