Z-Gly-Pro-AMC

Modify Date: 2024-01-02 20:34:00

Z-Gly-Pro-AMC Structure
Z-Gly-Pro-AMC structure
Common Name Z-Gly-Pro-AMC
CAS Number 68542-93-8 Molecular Weight 463.48300
Density 1.358g/cm3 Boiling Point 770.8ºC at 760 mmHg
Molecular Formula C25H25N3O6 Melting Point 96-102ºC(lit.)
MSDS N/A Flash Point 420ºC

 Use of Z-Gly-Pro-AMC


Z-Gly-Pro-AMC is a fluorogenic substrate. Z-Gly-Pro-AMC is hydrolyzed by prolyl endopeptidase to generate highly fluorescent 7-amido-4-methylcoumarin (HY-D0027). (λex=380 nm, λem=465 nm)[1].

 Names

Name benzyl N-[2-[(2S)-2-[(4-methyl-2-oxochromen-7-yl)carbamoyl]pyrrolidin-1-yl]-2-oxoethyl]carbamate
Synonym More Synonyms

 Z-Gly-Pro-AMC Biological Activity

Description Z-Gly-Pro-AMC is a fluorogenic substrate. Z-Gly-Pro-AMC is hydrolyzed by prolyl endopeptidase to generate highly fluorescent 7-amido-4-methylcoumarin (HY-D0027). (λex=380 nm, λem=465 nm)[1].
Related Catalog
In Vitro Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Z-Gly-Pro-AMC assay[1]: 1. Make a 5 μL of plasma sample is pre-incubated with 10 μL of 250 nM FAP inhibitor, 10 μL of 250 nM PREP inhibitor or 10 μL of 0.0025% (v/v) DMSO for 15 min at 37 ℃. 2. Add 35 μL pre-heated Z-Gly-Pro-AMC (380 μM diluted in buffer) to obtain a final concentration of 266 μM. 3. Fluorescence is measured kinetically for 30 min at 37 ℃.
References

[1]. Bracke A, et, al. The development and validation of a combined kinetic fluorometric activity assay for fibroblast activation protein alpha and prolyl oligopeptidase in plasma. Clin Chim Acta. 2019 Aug;495:154-160.

 Chemical & Physical Properties

Density 1.358g/cm3
Boiling Point 770.8ºC at 760 mmHg
Melting Point 96-102ºC(lit.)
Molecular Formula C25H25N3O6
Molecular Weight 463.48300
Flash Point 420ºC
Exact Mass 463.17400
PSA 117.95000
LogP 3.35910
Index of Refraction 1.635
Storage condition −20°C

 Safety Information

Safety Phrases S22;S24/25
WGK Germany 3

 Synonyms

Z-GP-AMC
Z-Gly-Pro-AMC
Z-Gly-propmecounh
N-Carbobenzoxyglycylprolyl-4-methylcoumarinyl amide
Z-Gly-pro-NH-Mec
MFCD00038082