Cresyl Violet acetate

Modify Date: 2024-01-02 11:35:06

Cresyl Violet acetate structure	Common Name	Cresyl Violet acetate
	CAS Number	10510-54-0	Molecular Weight	321.330
	Density	N/A	Boiling Point	468.3ºC
	Molecular Formula	C₁₈H₁₅N₃O₃	Melting Point	140-143 °C(lit.)
	MSDS	Chinese USA	Flash Point	237ºC

Use of Cresyl Violet acetate

Cresyl violet acetate is a red fluorescent stain, which can be used to stain neurons.

Name	cresyl violet acetate
Synonym	More Synonyms

Description	Cresyl violet acetate is a red fluorescent stain, which can be used to stain neurons.
Related Catalog	Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Neurological Disease
In Vitro	The estimated total number of SG neurons is 27,485±3251 and 26,705±1823 in the PV and Cresyl violet acetate stained sections, respectively. There is no significant difference between them (p=0.552). Therefore, Cresyl violet acetate staining is simpler and more cost effective when estimates neuronal number. Although PV stains spiral ganglion neurons (SGNs) with a greater intensity and provides a functional status, its tedious protocol limits its use for quantification. Total RC volume is estimated using probe and it is found that an average RC volume of 2.162±0.35 mm3 and 1.82±0.33 mm3 in Cresyl violet acetate staining and PV immunostaining sections, respectively. Volume of neurons is estimated using nucleator probe and it is 3487.63±951 μm3 and 3740.1±784 μm3 in Cresyl violet acetate staining and PV immunostaining sections, respectively. Similarly, volume of neuronal nucleus is also estimated using nucleator probe and it is found to be 131.68±50 μm3 and 126.51±33 μm3 in Cresyl violet acetate staining and PV immunostaining sections, respectively[1].
Cell Assay	Cochlear sections containing SGNs are placed in 24 wells plates containing PBS (pH 7.4) and stored at 4°C. The sections are then used for Cresyl violet acetate and immunohistochemical (IHC) staining. Every 7th section is stained with Cresyl violet acetate (1%), dehydrated with ascending grades of alcohol, cleared with xylene, mounted with DPX and observed under microscope. Approximately 12-13 Cresyl violet acetate staining sections from each specimen are used for stereology. None of these cases show any histopathological changes under the light microscope. Estimation of the total volume of the Rosenthal canal (RC), total number of SGNs (optical fractionator probe) and the volume of the soma and their nucleus (nucleator probe) is done with software[1].
References	[1]. Kaur C, et al. Comparison of unbiased stereological estimation of total number of cresyl violet stained neurons and parvalbumin positive neurons in the adult human spiral ganglion. J Chem Neuroanat. 2017 Jun 23. pii: S0891-0618(17)30037-6.

Boiling Point	468.3ºC
Melting Point	140-143 °C(lit.)
Molecular Formula	C₁₈H₁₅N₃O₃
Molecular Weight	321.330
Flash Point	237ºC
Exact Mass	321.111328
PSA	113.20000
LogP	3.91930
Vapour Pressure	6.07E-09mmHg at 25°C
Stability	Stable. Incompatible with strong oxidizing agents.

Cresyl Violet acetate MSDS(Chinese)

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes	Xi: Irritant;
Risk Phrases	R36/37/38
Safety Phrases	S37/39-S26
RIDADR	NONH for all modes of transport
WGK Germany	3

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