In Vitro |
NCT-504 dose not impair the intrinsic ATP-hydrolytic activity of PIP4Kγ in the absence of PI5P substrate[1]. NCT-504 does not inhibit PIP4Kbeta and weakly inhibits PIP4Kalpha phosphorylation of PI5P [1]. NCT-504 dose not inhibit PIP4Kbeta or PIP4Kalpha (IC50 between 50 μM and 100 μM) at 50 μM concentration[1]. NCT-504 elevates the levels of PI(3,5)P2, PI3P and PI5P in MEFs[1]. NCT-504 (10 μM; 12 hours) does not affect cell viability in MEFs[1]. NCT-504 (5 μM, 10 μM; 2 hours, 6 hours) elevates both the induction of autophagy as well as the rate of turnover of autophagic cargo[1]. NCT-504 treatment causes a robust increase in the formation of autolysosomes with only a modest elevation in autophagosomes[1]. NCT-504 increases autophagy flux and decreases huntingtin protein in 293A cells[1]. NCT-504 reduces mHtt protein levels in immortalized striatal cells from knock-in HD mice[1]. Western Blot Analysis[1] Cell Line: HEK293T cells Concentration: 5 μM, 10 μM Incubation Time: 2 hours, 6 hours Result: Induced autophagosome formation.
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