Ethidium bromide

Modify Date: 2025-08-20 16:15:13

Ethidium bromide structure	Common Name	Ethidium bromide
	CAS Number	1239-45-8	Molecular Weight	394.308
	Density	N/A	Boiling Point	N/A
	Molecular Formula	C₂₁H₂₀BrN₃	Melting Point	260-262 °C (dec.)(lit.)
	MSDS	Chinese USA	Flash Point	>100°C
	Symbol	GHS06, GHS08	Signal Word	Danger

Use of Ethidium bromide

Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.

Name	ethidium bromide
Synonym	More Synonyms

Description	Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). The preparation of agarose gel: 1． Agarose gels are commonly used in concentrations of 0.5% to 2.5% depending on the size of bands needed to be separated. 2． Mix the agarose powder with 1X TAE/TBE. 3． Microwave for 1-3 min until the agarose is completely dissolved (Caution: not overboil). 4． Make the solution cool down before solidification. 5． Add ethidium bromide (EtBr) to a final concentration of approximately 0.2-0.5 μg/mL (Stocks are generally 10 mg/ml, and require 5 µL stock/100 mL gel). 6． Ethidium bromide binds to the DNA and you could visualize the DNA under ultraviolet (UV) light. CAUTION: EtBr is a known mutagen. Please pay attention to strengthening protection.
References	[1]. P Stevenson, et al. Comparison of isometamidium chloride and homidium bromide as prophylactic drugs for trypanosomiasis in cattle at Nguruman, Kenya. Acta Trop. 1995 May;59(2):77-84. [2]. Sabnis, Ram Wasudeo (2010). Handbook of Biological Dyes and Stains: Synthesis and Industrial Application. Hoboken, NJ: Wiley. ISBN 978-0-470-40753-0.

Melting Point	260-262 °C (dec.)(lit.)
Molecular Formula	C₂₁H₂₀BrN₃
Molecular Weight	394.308
Flash Point	>100°C
Exact Mass	393.084045
PSA	55.92000
LogP	2.29820
Index of Refraction	1.4575 (20ºC)
Storage condition	2-8°C
Stability	Stable. Incompatible with strong oxidizing agents.
Water Solubility	40 g/L (25 ºC)

Ethidium bromide MSDS(Chinese)

CHEMICAL IDENTIFICATION

RTECS NUMBER :: SF7950000

CHEMICAL NAME :: Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide

CAS REGISTRY NUMBER :: 1239-45-8

LAST UPDATED :: 199712

DATA ITEMS CITED :: 79

MOLECULAR FORMULA :: C21-H20-N3.Br

MOLECULAR WEIGHT :: 394.35

WISWESSER LINE NOTATION :: T B666 HKJ EJ H2 IR& LZ &E &9/26

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 20 mg/kg

TOXIC EFFECTS :: Tumorigenic - active as anti-cancer agent

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Subcutaneous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 110 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: Sex chromosome loss and nondisjunction

TYPE OF TEST :: Specific locus test

TYPE OF TEST :: Dominant lethal test

TYPE OF TEST :: Micronucleus test

TYPE OF TEST :: DNA inhibition

TYPE OF TEST :: Sister chromatid exchange

MUTATION DATA

TEST SYSTEM :: Bird - chicken

DOSE/DURATION :: 847 ug/kg

REFERENCE :: 47JMAE "Cytogenetic Assays of Environmental Mutagens," Hsu, T.C., ed., Totowa, NJ, Allanheld, Osmun & Co., 1982 Volume(issue)/page/year: -,137,1982 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - A1168 No. of Facilities: 17 (estimated) No. of Industries: 1 No. of Occupations: 1 No. of Employees: 34 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - A1168 No. of Facilities: 7 (estimated) No. of Industries: 1 No. of Occupations: 3 No. of Employees: 21 (estimated)

Symbol	GHS06, GHS08
Signal Word	Danger
Hazard Statements	H302-H330-H341
Precautionary Statements	P260-P281-P284-P310
Personal Protective Equipment	Eyeshields;Faceshields;full-face particle respirator type N100 (US);Gloves;respirator cartridge type N100 (US);type P1 (EN143) respirator filter;type P3 (EN 143) respirator cartridges
Hazard Codes	T+:Verytoxic;
Risk Phrases	R21/22;R26;R36/37/38;R68
Safety Phrases	S36/37-S45-S36/37/39-S28A-S26-S22
RIDADR	UN 2811 6.1/PG 1
WGK Germany	3
RTECS	SF7950000
Packaging Group	I
Hazard Class	6.1
HS Code	29339990

Precursor 9
CAS#:93840-65-4 5-ethyl-3,8-din... CAS#:52671-22-4 3,8-bis((ethoxy... CAS#:2243-78-9 4-(4-aminopheny... CAS#:71857-96-0 amino-2 bis(eth...
CAS#:71857-87-9 nitro-2 bis(eth... CAS#:62895-39-0 3,8-DI-(ETHOXYC... CAS#:92-87-5 4-(4-aminopheny... CAS#:92-52-4 Biphenyl
CAS#:86-00-0 2-Nitrobiphenyl
DownStream 1
CAS#:104821-25-2 Dihydroethidium

HS Code	2933990090
Summary	2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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3,8-Diamino-5

MFCD00011724

3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide,EtBr,Homidium bromide

Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide (1:1)

Homidium bromide

3,8-Diamino-5-ethyl-6-phenylphenanthridin-5-ium bromide

3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide

EINECS 214-984-6

5-ethyl-6-phenylphenanthridin-5-ium-3,8-diamine,bromide

EthidiuM BroMide

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China
Product Name: ethidium bromide
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Price: ￥550/10 mM * 1 mL in DMSO

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Ethidium bromide

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