TPE-MI

Modify Date: 2024-01-09 10:30:37

TPE-MI structure	Common Name	TPE-MI
	CAS Number	1245606-71-6	Molecular Weight	441.520
	Density	1.2±0.1 g/cm3	Boiling Point	565.7±29.0 °C at 760 mmHg
	Molecular Formula	C₃₁H₂₃NO₂	Melting Point	N/A
	MSDS	USA	Flash Point	228.0±16.6 °C

Use of TPE-MI

TPE-MI (Tetraphenylethene maleimide) is a thiol probe for measuring unfolded protein load and proteostasis in cells. TPE-MI can report imbalances in proteostasis in induced pluripotent stem cell models of Huntington disease, as well as cells transfected with mutant Huntington exon 1 before the formation of visible aggregates. TPE-MI also detects protein damage following dihydroartemisinin treatment of the malaria parasitesPlasmodium falciparum [1][2].

Name	TPE-Thiol
Synonym	More Synonyms

Description	TPE-MI (Tetraphenylethene maleimide) is a thiol probe for measuring unfolded protein load and proteostasis in cells. TPE-MI can report imbalances in proteostasis in induced pluripotent stem cell models of Huntington disease, as well as cells transfected with mutant Huntington exon 1 before the formation of visible aggregates. TPE-MI also detects protein damage following dihydroartemisinin treatment of the malaria parasitesPlasmodium falciparum [1][2].
Related Catalog	Signaling Pathways >> Others >> Others Research Areas >> Neurological Disease
In Vitro	TPE-MI is inherently non-fluorescent until it is conjugated to a thiol via the maleimide. TPE-MI fluorescence is activated upon labelling free cysteine thiols, normally buried in the core of globular proteins that are exposed upon unfolding[1]. TPE-MI (50 µM; 0-60 min) exhibits a homogeneous cytoplasmic labelling pattern in live HeLa cells, with a lower level of labelling in the nucleus and apparent concentration in the region of the ER, which was anticipated as a major location for protein synthesis and folding[1]. At the high expression level, the mutant 97Q form of Httex1 was associated with an elevated TPE-MI fluorescence signal relative to a non-disease-causing 25Q form of Httex1[1]. TPE-MI consists of the aggregation-induced emission (AIE) fluorogen tetraphenylethane (TPE) and the thiol-reactive group maleimide (MI), thereby possessing both AIE phenomenon and selective thiol reactivity[2].
References	[1]. Chen MZ, et al. A thiol probe for measuring unfolded protein load and proteostasis in cells. Nat Commun. 2017;8(1):474. Published 2017 Sep 7. [2]. Hu Q, et al. In Situ Monitored Vortex Fluidic-Mediated Protein Refolding/Unfolding Using an Aggregation-Induced Emission Bioprobe. Molecules. 2021;26(14):4273. Published 2021 Jul 14.

Density	1.2±0.1 g/cm3
Boiling Point	565.7±29.0 °C at 760 mmHg
Molecular Formula	C₃₁H₂₃NO₂
Molecular Weight	441.520
Flash Point	228.0±16.6 °C
Exact Mass	441.172882
LogP	7.74
Vapour Pressure	0.0±1.5 mmHg at 25°C
Index of Refraction	1.665

RIDADR	NONH for all modes of transport

Simple Biosensor with High Selectivity and Sensitivity: Thiol-Specific Biomolecular Probing and Intracellular Imaging by AIE Fluorogen on a TLC Plate through a Thiol–Ene Click Mechanism Yang Liu, Yong Yu, Jacky W. Y. Lam, Yuning Hong, Mahtab Faisal, et al

Chemistry 16(28) , 8433-8438, (2010)

1-[4-[2-(4-Methylphenyl)-1,2-diphenylethenyl]phenyl]-1H-pyrrole-2,5-dione

1-{4-[(E)-2-(4-Methylphenyl)-1,2-diphenylvinyl]phenyl}-1H-pyrrole-2,5-dione

TPE-Thiol

1H-Pyrrole-2,5-dione, 1-[4-[(E)-2-(4-methylphenyl)-1,2-diphenylethenyl]phenyl]-