CU-CPT17e
Modify Date: 2025-08-27 11:12:12
CU-CPT17e structure
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Common Name | CU-CPT17e | ||
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| CAS Number | 2109805-75-4 | Molecular Weight | 504.49 | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | C27H24N2O8 | Melting Point | N/A | |
| MSDS | N/A | Flash Point | N/A | |
Use of CU-CPT17eCU-CPT17e is a multi-Toll-like receptor (TLR) agonist that activates TLR3, TLR8, and TLR9. |
| Name | CU-CPT17e |
|---|
| Description | CU-CPT17e is a multi-Toll-like receptor (TLR) agonist that activates TLR3, TLR8, and TLR9. |
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| Related Catalog | |
| Target |
TLR3 TLR8 TLR9 |
| In Vitro | CU-CPT17e shows strong NF-κB activation in TLR3, TLR8 and TLR9 HEK293 cells with EC50 values of 4.80±0.73, 13.5±0.58 and 5.66±0.17 μM, respectively. CU-CPT17e significantly improves the activity with 13.9±0.9 fold of NF-κB activation and an EC50 value of 4.8±0.7 μM. CU-CPT17e inhibits the proliferation of HeLa cancer cells by triggering apoptosis and arresting the cell cycle at the S phase. The induction of apoptosis by CU-CPT17e in HeLa cells is investigated. HeLa cells are cultured with increasing concentrations of CU-CPT17e or poly I:C or blank control (DMSO) for 24 h. Treatment with CU-CPT17e for 24 h at different concentrations (10 to 40 μM) results in an elevation of apoptotic cell population ranging from 10% to 17%, which is more effective than poly I:C at 5 μg/mL. These results suggest that the antiproliferative activity of CU-CPT17e against HeLa cells might result from its ability to directly induce apoptosis[1]. |
| Cell Assay | HeLa cells are seeded at a density of 3×105 cells/well in 6-well plates and allowed to attach for 24 h. After treatment of indicated concentrations of CU-CPT17e or poly I:C (5 μg/mL) for another 24 h, cells are harvested with 0.25% trypsin without EDTA and rinsed twice with PBS, then stained using a Annexin V-FITC apoptosis detection kit. Cells are analyzed with a BD Accuri C6 flow cytometer[1]. |
| References |
| Molecular Formula | C27H24N2O8 |
|---|---|
| Molecular Weight | 504.49 |
| Storage condition | 2-8℃ |