Hydroxystilbamidine bis(methanesulfonate)
Modify Date: 2024-01-13 17:15:28
Hydroxystilbamidine bis(methanesulfonate) structure
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Common Name | Hydroxystilbamidine bis(methanesulfonate) | ||
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| CAS Number | 223769-64-0 | Molecular Weight | 472.536 | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | C18H24N4O7S2 | Melting Point | N/A | |
| MSDS | Chinese USA | Flash Point | N/A | |
| Symbol |
GHS07 |
Signal Word | Warning | |
Use of Hydroxystilbamidine bis(methanesulfonate)Hydroxystilbamidine bis(methanesulfonate), a dye capable of binding to both DNA and RNA, has been found to be a powerful inhibitor of cellular ribonucleases. |
| Name | (4E)-4-{2-[4-(Diaminomethylene)-2,5-cyclohexadien-1-ylidene]ethylidene}-3-oxo-1,5-cyclohexadiene-1-carboximidamide methanesulfonate (1:2) |
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| Synonym | More Synonyms |
| Description | Hydroxystilbamidine bis(methanesulfonate), a dye capable of binding to both DNA and RNA, has been found to be a powerful inhibitor of cellular ribonucleases. |
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| Related Catalog | |
| In Vitro | It is found that the trypanocidal dye Hydroxystilbamidine bis(methanesulfonate) permits the recovery of mRNA after polysome released with Nonidet P-40 (NP-40). Sucrose gradient analysis of detergent-lysed postnuclear supernates is used to analyze the size distribution of NP-40-released polysomes. The heparin gradient shows some polyribosomes, whereas the Hydroxystilbamidine bis(methanesulfonate) gradient shows a remarkably large peak of very heavy polyribosomes. This peak is obtained reproducibly if Hydroxystilbamidine bis(methanesulfonate) is present before the addition of NP-40[1]. |
| In Vivo | Hydroxystilbamidine bis(methanesulfonate) is an effective suppressor of the plaque-forming cell (PFC) response when given before sheep erythrocytes (SRBC) stimulation. Hydroxystilbamidine bis(methanesulfonate) depresses the plaque response of the treated mice. Fewer PFC are observed in Hydroxystilbamidine bis(methanesulfonate)-treated mice throughout the experiment, but the level of suppression decreases with time. By day 14, the number of PFC observed in both the Hydroxystilbamidine bis(methanesulfonate) treated mice and the control group is essentially at the background level[2]. |
| Kinase Assay | Silkwork larvae are used in this study. Larvae on the fourth or fifth day of the fifth instar (~4.2 g body weight) are injected with 35 μL of a solution of 10 mg/mL cyclobeximidein H2O. After 5 min, the animals are immobilized in ice, and posterior silk glands are dissected and washed in ice-cold 0.15 M NaCl, 0.015 M Na citrate, 100 μg/mL cycloheximide. Washed glands from two larvae are placed in a homogenizer containing 4.7 mL of 40 mM triethanolamine-HCl, pH 7.5, 0.15 M sucrose, 0.1 M KCI, 3 mM MgCl2, 2 mM reduced glutathione, 10 μg/mL cycloheximide, 750 μg/mL Escherichia coli tRNA, and an appropriate concentration of RNase inhibitor (sodium heparin, 1.5 μg/mL orHydroxystilbamidine bis(methanesulfonate), 1.5 mM)[1]. |
| Animal Admin | Mice (six per group) are given various doses of Hydroxystilbamidine bis(methanesulfonate) (HSB) 3, 2, and 1 day before antigen. Other groups are given Hydroxystilbamidine bis(methanesulfonate) 1 or 2 days after the injection of antigen. Another group of mice receive antigen and Hydroxystilbamidine bis(methanesulfonate) simultaneously. A control group receives only antigen. The antigen dose consists of 2×108 sheep erythrocytes (SRBC). Four days after the injection of SRBC, the mice are sacrificed and spleens are removed and assayed for plaque-forming cell (PFC) by the plaque assay[2]. |
| References |
| Molecular Formula | C18H24N4O7S2 |
|---|---|
| Molecular Weight | 472.536 |
| Exact Mass | 472.108643 |
| Symbol |
GHS07 |
|---|---|
| Signal Word | Warning |
| Hazard Statements | H315-H319-H335 |
| Precautionary Statements | P261-P305 + P351 + P338 |
| Personal Protective Equipment | dust mask type N95 (US);Eyeshields;Gloves |
| RIDADR | NONH for all modes of transport |
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Brain Struct. Funct. , (2013) We have mapped the motor neurons (MNs) supplying the major hindlimb muscles of transgenic (C57/BL6J-ChAT-EGFP) and wild-type (C57/BL6J) mice. The fluorescent retrograde tracer Fluoro-Gold was injected... |
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Changes in projections to the inferior colliculus following early hearing loss in rats
Hear. Res. 287 , 57-66, (2012) The purpose of this study was to investigate the effects of early hearing loss on the anatomy of the central auditory system, specifically, the ascending projections to the inferior colliculus (IC). W... |
| (4E)-4-{2-[4-(Diaminomethylene)-2,5-cyclohexadien-1-ylidene]ethylidene}-3-oxo-1,5-cyclohexadiene-1-carboximidamide methanesulfonate (1:2) |
| 1,5-Cyclohexadiene-1-carboximidamide, 4-[2-[4-(diaminomethylene)-2,5-cyclohexadien-1-ylidene]ethylidene]-3-oxo-, (4E)-, methanesulfonate (1:2) |
| Hydroxystilbamidine bis(methanesulfonate) |