M344

Modify Date: 2024-01-20 04:06:07

M344 Structure
M344 structure
 Common Name M344
CAS Number 251456-60-7 Molecular Weight 307.388
Density 1.1±0.1 g/cm3 Boiling Point N/A
Molecular Formula C16H25N3O3 Melting Point 161℃
MSDS USA Flash Point N/A

 Use of M344


M344 (D 237) is an inhibitor of histone deacetylase (IC50=100 nM) and an inducer of terminal cell fifferentiation.

 Names

Name N-Hydroxy-7-(4-dimethylaminobenzoyl)aminoheptanamide
Synonym More Synonyms

 M344 Biological Activity

Description M344 (D 237) is an inhibitor of histone deacetylase (IC50=100 nM) and an inducer of terminal cell fifferentiation.
Related Catalog
Target

HDAC:100 nM (IC50)

In Vitro M344 is a potential histone deacetylase (HDAC) inhibitor. BRCA1 mRNA levels are determined by RT-PCR following exposure to increasing concentrations of the HDAC inhibitor M344 alone and in combination with Cisplatin in all 6 cell lines evaluated in this study. With increasing concentrations of M344, there is a dose dependant decrease in BRCA1 mRNA and treatment with both 1 and 5 μM concentrations of M344 resulting in a significant decrease in BRCA1 expression in all cell lines examined. M344 in combination with Cisplatin leads to a decrease in BRCA1 mRNA expression as compared to Cisplatin treatment alone in all cell lines with the exception of A2780s, which is recognized as having potent cytotoxicity to Cisplatin. In the MCF7 cell line, BRCA1 is down regulated at physiological doses of M344 (0.5 μM and 1 μM) but M344 does not have the same inhibitory effect on BRCA1 at the 5.0 μM dose. Co-treatment with Cisplatin and increasing concentrations of M344 reduces BRCA1 protein levels in all breast and ovarian cell lines examined[2].
Cell Assay The A2780s and A2780cp cell lines, the T-47D and OVCAR-4 cell lines. and the MCF7 and HCC1937cell lines, are maintained in Dulbecco's-MEM supplemented with 10% fetal bovine serum and 100 μg/mL penicillin-streptomycin. Unless otherwise described, cells are treated for 24 hrs with 2 μg/mL Cisplatin alone, and in combination with the HDAC inhibitor M344 at concentrations of 0.5, 1.0, or 5.0 μM. Phase contrast images are collected using the 10× objective of an Eclipse TE2000-U. Cell viability is measured by the MTT rapid colorimetric assay. Approximately 4,500 cells are seeded into each well of a 96-well flat bottom plate. The cells are incubated overnight to allow for cell attachment. Cells are then treated with Cisplatin in concentrations of 0-8 μg/mL alone or in combination with 1 μM of the HDAC inhibitor, M344. Forty eight hours following treatment, 42 μL of a 5 mg/mL MTT substrate solution in phosphate buffered saline (PBS) is added and incubated for up to 4 hrs at 37°C. The resulting violet formazan precipitate is solubilized by the addition of 82 μL of a 0.01 M HCl/10% SDS solution and plates are incubated overnight at 37°C. The plates are then analyzed on an MRX Microplate Reader at 570 nm to determine the optical density of the samples[2].
References

[1]. Jung M1, et al. Amide analogues of trichostatin A as inhibitors of histone deacetylase and inducers of terminal cell differentiation. J Med Chem. 1999 Nov 4;42(22):4669-79.

[2]. Weberpals JI, et al. The effect of the histone deacetylase inhibitor M344 on BRCA1 expression in breast and ovarian cancer cells. Cancer Cell Int. 2011 Aug 19;11(1):29.

 Chemical & Physical Properties

Density 1.1±0.1 g/cm3
Melting Point 161℃
Molecular Formula C16H25N3O3
Molecular Weight 307.388
Exact Mass 307.189606
PSA 81.67000
LogP 1.06
Appearance of Characters white to beige
Index of Refraction 1.558
Storage condition 2-8°C
Water Solubility DMSO: soluble10mg/mL, clear

 Safety Information

Hazard Codes Xi
RIDADR NONH for all modes of transport

 Articles7

More Articles
Loss-of-function HDAC8 mutations cause a phenotypic spectrum of Cornelia de Lange syndrome-like features, ocular hypertelorism, large fontanelle and X-linked inheritance.

Hum. Mol. Genet. 23(11) , 2888-900, (2014)

Cornelia de Lange syndrome (CdLS) is a multisystem genetic disorder with distinct facies, growth failure, intellectual disability, distal limb anomalies, gastrointestinal and neurological disease. Mut...

Acetylation regulates the stability of glutamate carboxypeptidase II protein in human astrocytes.

Biochem. Biophys. Res. Commun. 450(1) , 372-7, (2014)

Glutamate carboxypeptidase II (GCPII) is known to be implicated in brain diseases such as schizophrenia and bipolar disorder, and dramatically increases in prostate cancer. Here, we investigated the r...

HDAC inhibitors target HDAC5, upregulate microRNA-125a-5p, and induce apoptosis in breast cancer cells.

Mol. Ther. 23(4) , 656-66, (2015)

Histone deacetylase inhibitors (HDACi) are novel clinical anticancer drugs that inhibit HDAC gene expression and induce cell apoptosis in human cancers. Nevertheless, the detailed mechanism or the dow...

 Synonyms

4-(Dimethylamino)-N-[7-(hydroxyamino)-7-oxoheptyl]benzamide
4-(Dimethylamino)-N-(7-(hydroxyamino)-7-oxoheptyl)benzamide
Benzamide, 4-(dimethylamino)-N-[7-(hydroxyamino)-7-oxoheptyl]-
MFCD03453554
M 344
M344
Top Suppliers:I want be here

Get all suppliers and price by the below link:

M344 suppliers

Price: ￥567/5mg

Reference only. check more M344 price

Related Compounds: More...
M344
149647-78-9
Chemsrc provides M344(CAS#:251456-60-7) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of M344 are included as well.>> amp version: M344

Home | MSDS/SDS Database Search | Journals | Product Classification | Biologically Active Compounds | Selling Leads | About Us | Disclaimer

Copyright © 2024 ChemSrc All Rights Reserved