Methylene Blue

Modify Date: 2026-07-04 08:26:34

Methylene Blue Structure
Methylene Blue structure
 Common Name Methylene Blue
CAS Number 61-73-4 Molecular Weight 319.85200
Density 1.0 g/mL at 20 °C Boiling Point N/A
Molecular Formula C16H18ClN3S Melting Point 190 °C (dec.)(lit.)
MSDS Chinese Flash Point 45 °C
Symbol GHS02
GHS02		 Signal Word Warning

 Use of Methylene Blue


Methylene blue(Basic Blue 9) is used as a dye in chromoendoscopy, and is sprayed onto the mucosa of the gastrointestinal tract in order to identify dysplasia, or pre-cancerous lesions.

 Names

This table lists Chinese names, IUPAC names and various aliases of this chemical substance.

Name methylene blue
Synonym More Synonyms

 Methylene Blue Biological Activity

This table contains bioactivity, target information and biological‑assay experimental data of this compound.

Description Methylene blue(Basic Blue 9) is used as a dye in chromoendoscopy, and is sprayed onto the mucosa of the gastrointestinal tract in order to identify dysplasia, or pre-cancerous lesions.
Related Catalog
References

[1]. Methylene blue, From Wikipedia

 Chemical & Physical Properties

This table shows physicochemical parameters including melting point, boiling point, density, solubility and optical rotation. Missing data is marked as N/A.

Density 1.0 g/mL at 20 °C
Melting Point 190 °C (dec.)(lit.)
Molecular Formula C16H18ClN3S
Molecular Weight 319.85200
Flash Point 45 °C
Exact Mass 319.09100
PSA 47.38000
InChIKey CXKWCBBOMKCUKX-UHFFFAOYSA-M
SMILES CN(C)c1ccc2nc3ccc(=[N+](C)C)cc-3sc2c1.[Cl-]
Water Solubility 40 g/L (20 ºC)

 MSDS

This table provides MSDS information including hazard classification, first‑aid, fire‑fighting, spill handling, operation and storage instructions.

 Toxicological Information

This table summarizes toxicological test data, acute & chronic toxicity and ecological hazard parameters for this chemical.

CHEMICAL IDENTIFICATION

RTECS NUMBER :
SO5600000
CHEMICAL NAME :
3H-Phenothiazine, 7-(dimethylamino)-3-(methylimino)-, 3-methochloride
CAS REGISTRY NUMBER :
61-73-4
LAST UPDATED :
199710
DATA ITEMS CITED :
36
MOLECULAR FORMULA :
C16-H18-N3-S.Cl
MOLECULAR WEIGHT :
319.88
WISWESSER LINE NOTATION :
T C666 BS EY INJ EUK1&1 MN1&1 &G &421

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Unreported
SPECIES OBSERVED :
Human - infant
DOSE/DURATION :
15 mg/kg
TOXIC EFFECTS :
Lungs, Thorax, or Respiration - cyanosis Blood - other changes
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
1180 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intraperitoneal
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
180 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Subcutaneous
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
190 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
1250 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
3500 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intraperitoneal
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
150 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
77 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Mammal - dog
DOSE/DURATION :
500 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Mammal - dog
DOSE/DURATION :
50 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Primate - monkey
DOSE/DURATION :
10 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Mammal - cat
DOSE/DURATION :
41 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - rabbit
DOSE/DURATION :
1 gm/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Subcutaneous
SPECIES OBSERVED :
Rodent - guinea pig
DOSE/DURATION :
300 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Mammal - domestic
DOSE/DURATION :
42300 ug/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Oral
DOSE :
2500 mg/kg
SEX/DURATION :
female 1-22 day(s) after conception
TOXIC EFFECTS :
Reproductive - Fertility - post-implantation mortality (e.g. dead and/or resorbed implants per total number of implants)

MUTATION DATA

TYPE OF TEST :
DNA adduct
TEST SYSTEM :
Mammal - species unspecified Lymphocyte
DOSE/DURATION :
1830 nmol/L
REFERENCE :
BIPMAA Biopolymers. (John Wiley & Sons, Inc., 605 Third Ave., New York, NY 10158) V.1- 1963- Volume(issue)/page/year: 12,1099,1973 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - M1469 No. of Facilities: 4471 (estimated) No. of Industries: 35 No. of Occupations: 34 No. of Employees: 25916 (estimated) NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - T1406 No. of Facilities: 150 (estimated) No. of Industries: 2 No. of Occupations: 2 No. of Employees: 664 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - M1469 No. of Facilities: 5956 (estimated) No. of Industries: 29 No. of Occupations: 51 No. of Employees: 74208 (estimated) No. of Female Employees: 43305 (estimated)

 Safety Information

This table covers safety warnings, protective measures, incompatible substances and disposal guidelines for this compound.

Symbol GHS02
GHS02
Signal Word Warning
Hazard Statements H226
Personal Protective Equipment Eyeshields;Gloves
Hazard Codes Xn:Harmful
Risk Phrases R22;R41
Safety Phrases 26-39-36
RIDADR UN 1993 3/PG 3
WGK Germany 3
RTECS SP5740000
HS Code 2934300000

 Customs

This table shows customs‑related data including HS‑code, tariff and regulatory conditions for import and export.

HS Code 2934300000
Summary 2934300000. other compounds containing in the structure a phenothiazine ring-system (whether or not hydrogenated), not further fused. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

 Articles294

More Articles

This table lists relevant public references with title, journal and publication metadata for this compound.

Organization of pontine reticulospinal inputs to motoneurons controlling axial and limb muscles in the neonatal mouse.

J. Neurophysiol. 112(7) , 1628-43, (2014)

Using optical recording of synaptically mediated calcium transients and selective spinal lesions, we investigated the pattern of activation of spinal motoneurons (MNs) by the pontine reticulospinal pr...

High-throughput fluorescence-based screening assays for tryptophan-catabolizing enzymes.

J. Biomol. Screen. 19(9) , 1266-74, (2014)

Indoleamine 2,3-dioxygenase (IDO1) and tryptophan 2,3-dioxygenase (TDO) are two structurally different enzymes that have a different tissue distribution and physiological roles, but both catalyze the ...

Cytochrome C reacts with cholesterol hydroperoxides to produce lipid- and protein-derived radicals.

Biochemistry 54 , 2841-50, (2015)

Lipid peroxidation is a well-known process that has been implicated in many diseases. Recent evidence has shown that mitochondrial cholesterol levels are increased under specific conditions, making it...

 Synonyms

This table collects all English aliases, systematic names and CAS‑related synonyms of the compound.

Evan's Blue
SWISS BLUE
CI BASIC BLUE 9
BASIC BLUE 9
Methylene Blue
MFCD00012111
CI Direct Blue 53
EINECS 200-515-2
Chromosmon
CBLUENO.1
evans blue tetrasodium salt
MethyleneBlueGr

 Methylene Blue | FAQ

This section contains frequently‑asked‑questions about this compound, including basic parameters, properties, storage conditions and corresponding answers.

Q: What is the safety information for methylene blue?
A: Safety information for methylene blue: 26-39-36.
Q: What is the SMILES notation of methylene blue?
A: SMILES notation of methylene blue is CN(C)c1ccc2nc3ccc(=[N+](C)C)cc-3sc2c1.[Cl-].
Q: What are the uses of methylene blue?
A: Main uses of methylene blue: Methylene blue(Basic Blue 9) is used as a dye in chromoendoscopy, and is sprayed onto the mucosa of the gastrointestinal tract in order to identify dysplasia, or pre-cancerous lesions.
Q: What is the English name of methylene blue?
A: The English name of methylene blue is methylene blue.
Q: What is the melting point of methylene blue?
A: Melting point of methylene blue is 190 °C (dec.)(lit.).
Q: How is the water solubility of methylene blue?
A: Water solubility of methylene blue: 40 g/L (20 ºC).
Q: What is the polar surface area (PSA) of methylene blue?
A: Polar surface area (PSA) of methylene blue is 47.38000.
Q: What English synonyms does methylene blue have?
A: English synonyms of methylene blue: Evan's Blue, SWISS BLUE, CI BASIC BLUE 9, BASIC BLUE 9, Methylene Blue, MFCD00012111, CI Direct Blue 53, EINECS 200-515-2, Chromosmon, CBLUENO.1, evans blue tetrasodium salt, MethyleneBlueGr.
Q: What is the CAS number of methylene blue?
A: CAS number of methylene blue is 61-73-4.
Q: What is the InChIKey of methylene blue?
A: InChIKey of methylene blue is CXKWCBBOMKCUKX-UHFFFAOYSA-M.

 Methylene Bluefactory

This table lists manufacturers and suppliers of this compound, including vendor names and product supply reference information.

Shanghai Nianxing Industrial Co., Ltd
Dayang Chem (Hangzhou) Co., Ltd.
Henan Tianfu Chemical Co., Ltd.
Zhongshan Xingrui Chemical Co. , Ltd.
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Chemsrc provides Methylene Blue(CAS#:61-73-4) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Articles of Methylene Blue are included as well.>> amp version: Methylene Blue

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