Nile Red

Modify Date: 2025-08-24 22:19:43

Nile Red Structure
Nile Red structure
Common Name Nile Red
CAS Number 7385-67-3 Molecular Weight 318.369
Density 1.2±0.1 g/cm3 Boiling Point 484.7±45.0 °C at 760 mmHg
Molecular Formula C20H18N2O2 Melting Point 203-205 °C(lit.)
MSDS Chinese USA Flash Point 246.9±28.7 °C

 Use of Nile Red


Nile Red is a strongly fluorescent stain in the presence of a hydrophobic environment for the detection of intracellular lipid droplets.

 Names

Name nile red dye
Synonym More Synonyms

 Nile Red Biological Activity

Description Nile Red is a strongly fluorescent stain in the presence of a hydrophobic environment for the detection of intracellular lipid droplets.
Related Catalog
References

[1]. Greenspan P, et al. Nile red: a selective fluorescent stain for intracellular lipid droplets. J Cell Biol. 1985 Mar;100(3):965-73.

 Chemical & Physical Properties

Density 1.2±0.1 g/cm3
Boiling Point 484.7±45.0 °C at 760 mmHg
Melting Point 203-205 °C(lit.)
Molecular Formula C20H18N2O2
Molecular Weight 318.369
Flash Point 246.9±28.7 °C
Exact Mass 318.136841
PSA 46.34000
LogP 3.65
Vapour Pressure 0.0±1.2 mmHg at 25°C
Index of Refraction 1.644
InChIKey VOFUROIFQGPCGE-UHFFFAOYSA-N
SMILES CCN(CC)c1ccc2nc3c4ccccc4c(=O)cc-3oc2c1
Stability Stable. Incompatible with strong oxidizing agents.

 Safety Information

Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Safety Phrases S24/25
RIDADR NONH for all modes of transport
WGK Germany 3
HS Code 2933990090

 Synthetic Route

~%

Nile Red Structure

Nile Red

CAS#:7385-67-3

Literature: Ionescu, Andreea; Godbert, Nicolas; Crispini, Alessandra; Termine, Roberto; Golemme, Attilio; Ghedini, Mauro Journal of Materials Chemistry, 2012 , vol. 22, # 44 p. 23617 - 23626

~24%

Nile Red Structure

Nile Red

CAS#:7385-67-3

Literature: Park, So-Yeon; Kubota, Yasuhiro; Funabiki, Kazumasa; Shiro, Motoo; Matsui, Masaki Tetrahedron Letters, 2009 , vol. 50, # 10 p. 1131 - 1135

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Nile Red Structure

Nile Red

CAS#:7385-67-3

Literature: Justus Liebigs Annalen der Chemie, , vol. 289, p. 94

 Customs

HS Code 2933990090
Summary 2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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 Nile RedBioassay

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Name: Primary cell-based high-throughput screening assay for identification of compounds th...
Source: Johns Hopkins Ion Channel Center
Target: regulator of G-protein signaling 4 isoform 2 [Homo sapiens]
External Id: JHICC_RGS_Act_HTS
Name: QFRET-based biochemical primary high throughput screening assay to identify exosite i...
Source: The Scripps Research Institute Molecular Screening Center
Target: disintegrin and metalloproteinase domain-containing protein 17 preproprotein [Homo sapiens]
External Id: ADAM17_INH_QFRET_1536_1X%INH PRUN
Name: qHTS Assay for Inhibitors of the Phosphatase Activity of Eya2
Source: NCGC
Target: eyes absent homolog 2 isoform a [Homo sapiens]
External Id: EYA2477
Name: uHTS identification of small molecule activators of the adaptive arm of the Unfolded ...
Source: Burnham Center for Chemical Genomics
Target: N/A
External Id: BCCG-A405-UPR-XBP1-PrimaryAgonist-Assay
Name: qHTS for Inhibitors of human tyrosyl-DNA phosphodiesterase 1 (TDP1): qHTS in cells in...
Source: NCGC
Target: TDP1 protein [Homo sapiens]
External Id: TDP1100
Name: qHTS for Inhibitors of human tyrosyl-DNA phosphodiesterase 1 (TDP1): qHTS in cells in...
Source: NCGC
Target: TDP1 protein [Homo sapiens]
External Id: TDP1101
Name: Primary cell-based high-throughput screening for identification of compounds that all...
Source: Johns Hopkins Ion Channel Center
Target: MAS-related GPR member X1 [Homo sapiens]
External Id: JHICC_MrgX1_AlloAgonist_Primary
Name: Primary cell-based high-throughput screening for identification of compounds that ant...
Source: Johns Hopkins Ion Channel Center
Target: MAS-related GPR member X1 [Homo sapiens]
External Id: JHICC_MrgX1_Antagonist_Primary
Name: Bursicon-induced LGR2 mediated cAMP production in LGR-2/CRE6x-Luciferase co-transfect...
Source: Broad Institute
Target: N/A
External Id: Bursicon-induced LGR2 mediated cAMP production in LGR-2/CRE6x-Luciferase co-transfected HEK293 cells Inhibition - 7011-01_Antagonist_SinglePoint_HTS_Activity
Name: High throughput screen for small molecule inhibitors of a hypoxia-regulated fluoresce...
Source: ICCB-Longwood/NSRB Screening Facility, Harvard Medical School
Target: N/A
External Id: HMS1149-MLP
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 Synonyms

Nile Red
9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one
9-(Diethylamino)-5H-benzo(a)phenoxazin-5-one
DCC impregnated tablets
Nile Blue A Oxazone
MFCD00011639
9-(Diethylamino)benzo(a)phenoxazin-5(5H)-one
9-(diethylamino)benzo[a]phenoxazin-5-one
EINECS 230-966-0
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