FLAG Peptide TFA salt

Modify Date: 2024-01-02 21:47:45

FLAG Peptide TFA salt structure	Common Name	FLAG Peptide TFA salt
	CAS Number	98849-88-8	Molecular Weight	1012.97000
	Density	N/A	Boiling Point	N/A
	Molecular Formula	C₄₁H₆₀N₁₀O₂₀	Melting Point	N/A
	MSDS	N/A	Flash Point	N/A

Use of FLAG Peptide TFA salt

FLAG peptide is an eight amino acids peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) with an enterokinase-cleavage site; designed for antibody-mediated identification and purification of recombinant proteins.

Name	L-Lysine, L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl
Synonym	More Synonyms

Description	FLAG peptide is an eight amino acids peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) with an enterokinase-cleavage site; designed for antibody-mediated identification and purification of recombinant proteins.
Related Catalog	Signaling Pathways >> Others >> Others Research Areas >> Inflammation/Immunology Peptides
In Vitro	Fusion protein technology has become an important tool for solving numerous problems linked to recombinant protein production. The properties of the additional tag facilitate identification and provide a one-step purification procedure of the fusion protein by passing cell extracts or supernatants through columns of an appropriate matrix. FLAG peptide allows elution under non-denaturing conditions. Several antibodies against FLAG peptide have been developed. One antibody, M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAGe peptide. Antibodies M2 and M5 are applied in this procedure[1]. The Flag-tag is first described as a calcium-dependent epitope of a monoclonal antibody. It is a highly acidic octapeptide which can be N-terminally fused to the protein of interest. As a very hydrophilic peptide the Flag–tag has a high surface probability. Flag-fusion proteins can be captured by an immunoaffinity column in the presence of Ca2+ and eluted byEDTA at low concentrations, neutral pH and thus, nearly physiological conditions[2].
References	[1]. Einhauer A, et al. The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 2001 Oct 30;49(1-3):455-65. [2]. Schuster M, et al. Protein expression in yeast; comparison of two expression strategies regarding proteinmaturation. J Biotechnol. 2000 Dec 28;84(3):237-48.

Molecular Formula	C₄₁H₆₀N₁₀O₂₀
Molecular Weight	1012.97000
Exact Mass	1012.40000
PSA	525.79000
Storage condition	-20°C

FLAG Peptide

FLAG EPITOPE PEPTIDE

Trypsinogen (human pancreas isoenzyme 2 activation peptide), 1-L-aspartic acid-2-L-tyrosine-3-L-lysine-

FLAG tag Peptide

ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS

GL Biochem (Shanghai) Ltd.
China
Product Name: ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS
Price: $Inquiry/1g
Purity: 98.0%
Stocking Period: Inquiry
Contact: Jackie Yang

Zhejiang Hangyu API Co., Ltd
China
Product Name: FLAG peptide
Price: $Inquiry/1kg
Purity: 99.0%
Stocking Period: 1 Day
Contact: Vinnie

Get all suppliers and price by the below link:

FLAG Peptide TFA salt suppliers

Price: $50/1mg

Reference only. check more FLAG Peptide TFA salt price