7α,25-Dihydroxycholesterol

7α,25-Dihydroxycholesterol用途

7α, 25-dihydroxycholesterol (7α,25-OHC) 是一种孤儿 GPCR 受体 EBI2 (GPR183) 的有效选择性激动剂和内源性配体。7α, 25-dihydroxycholesterol 对激活 EBI2 有很强的作用 (EC50=140 pM; Kd=450 pM)。7α, 25-dihydroxycholesterol 可作为一种趋化因子,指导 B 细胞、T 细胞和树突状细胞的迁移。

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7α,25-Dihydroxycholesterol名称

[ CAS 号 ]:
64907-22-8

[ 中文名 ]:
7α,25-二羟基胆固醇

[ 英文名 ]:
(3β,7α)-Cholest-5-ene-3,7,25-triol

[英文别名 ]:

Cholest-5-ene-3,7,25-triol, (3β,7α)-
7.α.,25-dihydroxy Cholesterol
(3β,7α)-Cholest-5-ene-3,7,25-triol

7α,25-Dihydroxycholesterol生物活性

[ 描述 ]:

[ 相关类别 ]:

研究领域 >> 炎症/免疫

信号通路 >> G 蛋白偶联受体/G 蛋白 >> EBI2/GPR183

[ 靶点 ]

Human Endogenous Metabolite

[体外研究]

在体外,7α,25二羟基胆甾醇(7α,25-OHC)刺激EBI2表达的小鼠B和T细胞在500 pm左右具有半最大有效浓度值,但对EBI2缺陷细胞没有影响[1 ]。

[体内研究]

经7α,25-二羟胆固醇(1μM；1.5小时)脱敏的EBI2缺陷B细胞或正常B细胞显示到脾脏滤泡区的归巢减少[1]。

[参考文献]

[1]. Liu C, et al. Oxysterols direct B-cell migration through EBI2. Nature. 2011 Jul 27;475(7357):519-23.

[2]. Hannedouche S, et al. Oxysterols direct immune cell migration via EBI2. Nature. 2011 Jul 27;475(7357):524-7.

7α,25-Dihydroxycholesterol物理化学性质

[ 密度 ]:
1.1±0.1 g/cm3

[ 沸点 ]:
548.5±35.0 °C at 760 mmHg

[ 熔点 ]:
235 - 236 °C

[ 分子式 ]:
C₂₇H₄₆O₃

[ 分子量 ]:
418.652

[ 闪点 ]:
230.5±20.5 °C

[ 精确质量 ]:
418.344696

[ PSA ]:
60.69000

[ LogP ]:
5.75

[ 蒸汽压 ]:
0.0±3.3 mmHg at 25°C

[ 折射率 ]:
1.550

[ 储存条件 ]:
2-8°C，密闭，干燥

7α,25-DihydroxycholesterolMSDS

详细MSDS(安全技术说明书)

7α,25-Dihydroxycholesterol安全信息

[ 危险品运输编码 ]:
NONH for all modes of transport

7α,25-Dihydroxycholesterol文献

Detection of dihydroxycholesterols in human plasma using HPLC-ESI-MS/MS.

Steroids 99 , 131-8, (2015)

We report a straightforward sample preparation procedure and a direct liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method for the analysis of 7alpha,25-dihydro...

Studies on the relationships between 7 alpha-hydroxylation and the ability of 25- and 27-hydroxycholesterol to suppress the activity of HMG-CoA reductase.

Biochim. Biophys. Acta 1344(3) , 241-9, (1997)

The metabolism of 25-hydroxycholesterol in different cell types was studied and the role of 7 alpha-hydroxylation for the effect of 25-hydroxycholesterol on the activity of HMG-CoA reductase was deter...

Molecular characterization of oxysterol binding to the Epstein-Barr virus-induced gene 2 (GPR183).

J. Biol. Chem. 287(42) , 35470-83, (2012)

Oxysterols are oxygenated cholesterol derivates that are emerging as a physiologically important group of molecules. Although they regulate a range of cellular processes, only few oxysterol-binding ef...

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