梭菌蛋白酶 来源于溶组织梭菌

FEMS Immunol. Med. Microbiol. 45(2) , 137-42, (2005)

Clostridium histolyticum culture supernatant contains numerous enzymes, which exert a cytotoxic effect on host cells. This includes lethal toxin, clostripain and high-potassium-sensitive toxin. Since the number of C. histolyticum infections increased during t...

Protein Expr. Purif. 76(1) , 83-9, (2011)

Clostripain (CLO) produced by Clostridium histolyticum is an arginine-specific endopeptidase with the potential for applicability to diverse medical and industrial uses. In this study, we developed an expression system allowing high-level production and effic...

J. Am. Chem. Soc. 127(49) , 17132-3, (2005)

We demonstrate that proteases can catalyze the ligation of peptidomimetic oligomers. The enzyme clostripain was used to facilitate the native ligation of N-substituted glycine oligomers, or peptoids. In addition to mediating the efficient condensation of two ...

J. Neurochem. 104(5) , 1404-14, (2008)

Neurotrauma, as in the case of traumatic brain injury, promotes protease over-activation characterized by the select fragmentation of brain proteins. The resulting polypeptides are indicators of biochemical processes, which can be used to study post-injury dy...

Biochemistry 44(18) , 6958-70, (2005)

In response to extracellular signals, G protein-coupled receptors (GPCRs) catalyze guanine nucleotide exchange on Galpha subunits, enabling both activated Galpha and Gbetagamma subunits to target downstream effector enzymes. One target of Gbetagamma is G prot...

J. Biol. Chem. 280(39) , 33687-92, (2005)

Ntcp is a phosphoprotein, and its translocation by cAMP to the plasma membrane is associated with dephosphorylation. However, the phosphorylation site(s) of Ntcp is not known. Thus, the aim of the present study was to determine the potential Ntcp phosphorylat...

Angew. Chem. Int. Ed. Engl. 42(6) , 677-9, (2003)

Angew. Chem. Int. Ed. Engl. 41(15) , 2735-8, (2002)

J. Biol. Chem. 277(36) , 33041-8, (2002)

DNA polymerase (gp43) of phage T4 plays two biological roles, one as an essential DNA binding replication enzyme and the other as an mRNA-specific autogenous translational repressor. Binding of T4 gp43 to its mRNA target (translational operator RNA) interfere...

Microbiology 156(Pt 2) , 561-9, (2010)

Clostridium perfringens produces a homologue of clostripain (Clo), the arginine-specific endopeptidase of Clostridium histolyticum. To determine the biochemical and biological properties of the C. perfringens homologue (Clp), it was purified from the culture ...