吡喃糖氧化酶 来源于革盖菌属结构式
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常用名 | 吡喃糖氧化酶 来源于革盖菌属 | 英文名 | Pyranose Oxidase from Coriolus |
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CAS号 | 37250-80-9 | 分子量 | N/A | |
密度 | N/A | 沸点 | N/A | |
分子式 | N/A | 熔点 | N/A | |
MSDS | 美版 | 闪点 | N/A |
A new set up for multi-analyte sensing: at-line bio-process monitoring.
Biosens. Bioelectron. 26(11) , 4532-7, (2011) A multi-analyte sensing device is described, for simultaneous at-line monitoring of glucose, ethanol, pO₂-value and cell density. It consists of a dual biosensor, a modified microscope and a fiber optical pO₂-sensor that are integrated into a flow analysis (F... |
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Enzyme-catalyzed O2 removal system for electrochemical analysis under ambient air: application in an amperometric nitrate biosensor.
Anal. Chem. 84(5) , 2141-6, (2012) Electroanalytical procedures are often subjected to oxygen interferences. However, achieving anaerobic conditions in field analytical chemistry is difficult. In this work, novel enzymatic systems were designed to maintain oxygen-free solutions in open, small ... |
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Enzymatic oxygen scavenging for photostability without pH drop in single-molecule experiments.
ACS Nano 6(7) , 6364-9, (2012) Over the past years, bottom-up bionanotechnology has been developed as a promising tool for future technological applications. Many of these biomolecule-based assemblies are characterized using various single-molecule techniques that require strict anaerobic ... |
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Resolution doubling in 3D-STORM imaging through improved buffers.
PLoS ONE 8(7) , e69004, (2013) Super-resolution imaging methods have revolutionized fluorescence microscopy by revealing the nanoscale organization of labeled proteins. In particular, single-molecule methods such as Stochastic Optical Reconstruction Microscopy (STORM) provide resolutions d... |
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Carbohydrate oxidase, a novel enzyme from Polyporus obtusus. II. Specificity and characterization of reaction products.
Biochim. Biophys. Acta 167(3) , 501-10, (1968)
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Pyranose dehydrogenases: biochemical features and perspectives of technological applications.
Appl. Microbiol. Biotechnol. 85(4) , 837-48, (2010) Pyranose dehydrogenase is a fungal flavin-dependent sugar oxidoreductase which is structurally and catalytically related to fungal pyranose oxidase and cellobiose dehydrogenase and probably fulfills similar biological functions in lignocellulose breakdown. It... |
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Identification of a catalytic base for sugar oxidation in the pyranose 2-oxidase reaction.
ChemBioChem. 12(17) , 2577-86, (2011) Pyranose 2-oxidase (P2O) catalyzes the oxidation of aldopyranoses to form 2-keto sugars and H(2)O(2) . In this study, the mechanistic role of the conserved residues His548 and Asn593 in P2O was investigated by using site-directed mutagenesis, transient kineti... |
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Heterologous expression and biochemical characterization of novel pyranose 2-oxidases from the ascomycetes Aspergillus nidulans and Aspergillus oryzae.
Appl. Microbiol. Biotechnol. 93(3) , 1157-66, (2012) A gene encoding a pyranose 2-oxidase (POx; pyranose/oxygen 2-oxidoreductase; glucose 2-oxidase; EC 1.1.3.10) was identified in the genome of the ascomycete Aspergillus nidulans. Attempts to isolate POx directly from A. nidulans cultures or to homologously ove... |
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Controlled feeding of hydrogen peroxide as oxygen source improves production of 5-ketofructose from L-sorbose using engineered pyranose 2-oxidase from Peniophora gigantea.
Biotechnol. Bioeng. 109(11) , 2941-5, (2012) 5-Keto-D-fructose is a useful starting material for the synthesis of pyrrolidine iminosugars. It can be prepared by regioselective oxidation of L-sorbose using pyranose 2-oxidase (P2Ox) and O(2) as a cosubstrate. As the solubility of O(2) in aqueous solution ... |
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Thermostable variants of pyranose 2-oxidase showing altered substrate selectivity for glucose and galactose.
J. Agric. Food Chem. 58(6) , 3465-71, (2010) The homotetrameric flavoprotein pyranose 2-oxidase (P2Ox) has several proposed biotechnological applications, among others as a biocatalyst for carbohydrate transformations toward higher-value products. To improve some of the catalytic properties of P2Ox from... |