对硝基苯基 α-D-吡喃木糖苷

Appl. Environ. Microbiol. 76(15) , 5247-53, (2010)

The soft rot fungus Penicillium purpurogenum grows on a variety of natural substrates and secretes various isoforms of xylanolytic enzymes, including three arabinofuranosidases. This work describes the biochemical properties as well as the nucleotide and amin...

Biochim. Biophys. Acta 1794(1) , 144-58, (2009)

Catalysis and inhibitor binding by the GH43 beta-xylosidase are governed by the protonation states of catalytic base (D14, pK(a) 5.0) and catalytic acid (E186, pK(a) 7.2) which reside in subsite -1 of the two-subsite active site. Cationic aminoalcohols are sh...

J. Microbiol. Biotechnol. 20(12) , 1711-6, (2010)

A gene encoding the beta-xylosidase/alpha-arabinofuranosidase (XylC) of Paenibacillus woosongensis was cloned into Escherichia coli. This xylC gene consisted of 1,425 nucleotides, encoding a polypeptide of 474 amino acid residues. The deduced amino acid seque...

Carbohydr. Res. 338(18) , 1881-90, (2003)

An endo-beta-1,4-xylanase (1,4-beta-D-xylan xylanoxydrolase, EC 3.2.1.8) present in culture filtrates of Sporotrichum thermophile ATCC 34628 was purified to homogeneity by Q-Sepharose and Sephacryl S-200 column chromatographies. The enzyme has a molecular mas...

Lett. Appl. Microbiol. 54(2) , 79-87, (2012)

  To characterize the duel activities of a glycosyl hydrolase family 3 β-glucosidase/xylosidase from rumen bacterial metagenome and to investigate the capabilities of its β-d-xylosidase activities for saccharification of hemicellulosic xylans.  A β-glucosidas...

Am. J. Physiol. Cell Physiol. 283(2) , C438-45, (2002)

Sulfated proteoglycans have been proposed to play a role in the sorting and storage of secretory proteins in exocrine secretory granules. Rat parotid acinar cells expressed a 40- to 60-kDa proteoglycan that was stored in secretory granules. Treatment of the t...

Biochim. Biophys. Acta 1770(4) , 565-70, (2007)

Positional specificity of NodB-like domain of a multidomain xylanase U from Clostridium thermocellum (CtAxe) was investigated. Of three monoacetates of 4-nitrophenyl beta-d-xylopyranoside the acetylxylan esterase domain showed a clear preference for the 2-ace...

Biochim. Biophys. Acta 1472(1-2) , 250-61, (1999)

In order to determine the signal transduction pathways involved in the regulation of proteoglycan (PG) synthesis in immature rat Sertoli cells (SC), we have examined the effect of the tumor promoter phorbol ester PMA (phorbol myristate acetate) on [35S]sulfat...

Eur. J. Cell Biol. 82(1) , 19-29, (2003)

Serglycin is known as a secretory granule proteoglyean in hematopoietic cells. In this study we identified a high-molecular-weight molecule in aggregated content proteins of zymogen granules of pancreatic acinar cells. The amino acid composition of the isolat...

Biochim. Biophys. Acta 1770(4) , 638-48, (2007)

FSH-induced upregulation of cAMP-PDE4 activities was decreased in cultured Sertoli cells when alteration of cell proteoglycans (PGs) metabolism was simultaneously induced either by para-nitrophenyl beta-d-xyloside (PNPX) or by sodium chlorate. This effect was...